Pdgfrα clone apa5

Manufactured by BioLegend

PDGFRα (clone APA5) is a recombinant monoclonal antibody that recognizes the alpha subunit of the platelet-derived growth factor receptor (PDGFRα). This antibody is suitable for flow cytometry and other immunoassay applications.

Automatically generated - may contain errors

Lab products found in correlation

Close spelling variants of this product

PDGFRα (9 citations)

2 protocols using pdgfrα clone apa5

Tumor-Infiltrating Immune Cell Profiling

Check if the same lab product or an alternative is used in the 5 most similar protocols

Single-cell suspensions of transplanted tumors were obtained by digestion with 1 mg/ml Collagenase I (Sigma) and 1 mg/ml Dispase II (Roche) in RPMI containing 5 % FBS for 45 min at 37°C under shaking condition. The cell suspension was passed through a 70 μm nylon mesh and centrifuged. After red blood cell lysis, Fc receptors were blocked with anti-mouse FcR antibody (clone 93, Biolegend) for 15 min at a 1:100 dilution on ice. Then, cells were stained with anti-CD45 (clone 30-F11, Biolegend), CD11b (clone M1/70, Biolegend), Gr-1 (clone RB6-8C5, Biolegend), F4/80 (clone BM8, Biolegend), PDGFRα (clone APA5; Biolegend), CD206 (clone C068C2, Biolegend), CD11c (clone N418, Biolegend) and MHC II (I-A/I-E, clone M5/114.15.2, Biolegend) antibodies for 30 min at a 1:100 dilution on ice. For analysis of Treg cells in the tumor, cells were stained with anti-CD4 (clone GK1.5, Biolegend, 1:100), Foxp3 (clone FJK-16s, eBioscience, 1:50) antibodies using a Foxp3 staining buffer kit following the instructions (eBioscience). Samples were acquired on a Canto II flow cytometer (BD Biosciences) and the data were analyzed with FlowJo software.

Zhu Y., Zhang L., Zha H., Yang F., Hu C., Chen L., Guo B, & Zhu B. (2017). Stroma-derived Fibrinogen-like Protein 2 Activates Cancer-associated Fibroblasts to Promote Tumor Growth in Lung Cancer. International Journal of Biological Sciences, 13(6), 804-814.

+ Open protocol

+ Expand

Characterizing Cancer-Associated Fibroblasts

Check if the same lab product or an alternative is used in the 5 most similar protocols

FACs analysis of fibroblasts was performed using a panel of typical CAF markers; podoplanin (clone 8.1.1), PDGFRα (clone APA5) and β (clone APB5) (all 1:300, all BioLegend), FAP-α (1:50, AF3715; R&D Systems); and markers to exclude immune cells (MHCII; clone KH74 and CD45; clone 30-F11), epithelial cells (EpCAM, clone G8.8; Biolegend) and endothelial cells (CD31, clone 390; BioLegend). Morphological characteristics assed and visualised using an EVOS microscope and functional analysis was measured in terms of collagen gel contraction capacity. 1.5 × 10⁵ cells were seeded into 2 mg ml⁻¹ collagen gel (BD Biosciences) and detached from the sides of 24 well plates. Gel contraction was imaged over time and quantified as percentage area change over time. CAFs displayed typical CAF morphology, marker profiles and functionality were utilised (Supplementary Fig. 1).

Lakins M.A., Ghorani E., Munir H., Martins C.P, & Shields J.D. (2018). Cancer-associated fibroblasts induce antigen-specific deletion of CD8+ T Cells to protect tumour cells. Nature Communications, 9, 948.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!