The fresh plant of Fumaria indica was collected from the field area of New Delhi, India in the month of December 2018 and was authenticated by taxonomist, College of Science, King Saud University, Riyadh, Saudi Arabia. Standard protopine (purity: 99.5% w/w) was purchased from Sigma Aldrich, United States. All solvents used were of chromatography grade and all chemicals used were of analytical reagent grade. Precoated silica gel 60 F254 HPTLC plates were purchased from Merck, Darmstadt, Germany.
Precoated silica gel 60 f254 hptlc plates
Manufactured by Merck Group
Sourced in Germany
Precoated silica gel 60 F254 HPTLC plates are a type of thin-layer chromatography (TLC) substrate used for high-performance thin-layer chromatography (HPTLC) analysis. These plates are coated with silica gel 60 and contain a fluorescent indicator (F254) that allows for the visualization of separated compounds under UV light.
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2 protocols using precoated silica gel 60 f254 hptlc plates
1
Quantitative Analysis of Fumaria indica
2
High-Performance Thin-Layer Chromatography Analysis
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A CAMAG automatic TLC sampler 4 (CAMAG, Muttenz, Switzerland) and a CAMAG 25 μl sample syringe with CAMAG ATS 4 applicator were used for sample application. Pre-coated silica gel 60 F254 HPTLC plates (E. Merck, Darmstadt, Germany) of 0.2 mm thickness was used as the stationary phase. After the spotting of samples, the plates were developed in a twin trough chamber pre saturated with mobile phase and run to solvent front 90 mm under room temperature. Photographs of the developed plates were performed employing a CAMAG TLC visualizer at 254 nm and 366 nm. Densitometry scanning of the developed plates were performed using CAMAG TLC scanner in 366 nm and 254 nm with a slit dimension of 6 × 0.30 mm and better quantification, and data analysis was worked out by the CAMAG winCATS integrated software.
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