SVF or BMDM cultures were treated with human Fc-receptor block (BD Pharmingen) before staining with anti-human or anti-mouse fluorochrome-conjugated monoclonal antibodies specific for CD3ε (HIT3a, 1:500), CD9 (HI9a, 1:500), CD11b (M1/70, 1:500), CD11c (3.9, 1:500), CD14 (M5E2, 1:500), CD15 (W6D3, 1:500), CD16 (3G8, 1:500), CD45 (HI30, 1:500), and CD20 (HIB19, 1:500). Cells were also stained with DAPI (2 ng/mL) to exclude dead cells. Compensation was performed with cells or OneComp eBeads beads (Thermo Fisher Scientific) or cells as appropriate. Samples were analyzed with a BD LSR II and running DiVa software (BD Bioscience) and analyzed with FlowJo software (version 10.8). A minimum of 100,000 cells were employed for each analysis.
Human fc receptor block
Manufactured by BD
The Human Fc-receptor block is a laboratory reagent used in various immunological and biochemical assays. It functions by blocking the binding of Fc-containing molecules, such as antibodies, to Fc receptors expressed on cells. This allows for the specific detection or isolation of target analytes without interference from non-specific Fc-mediated interactions.
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Lab products found in correlation
2 protocols using human fc receptor block
1
Multiparametric Flow Cytometry Analysis
2
Multiparametric Flow Cytometry Analysis
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SVF or bone marrow–derived macrophage (BMDM) cultures were treated with human Fc-receptor block (BD Pharmingen) before staining with antihuman or antimouse fluorochrome-conjugated monoclonal antibodies specific for CD3ε (HIT3a, 1:500), CD9 (HI9a, 1:500), CD11b (M1/70, 1:500), CD11c (3.9, 1:500), CD14 (M5E2, 1:500), CD15 (W6D3, 1:500), CD16 (3G8, 1:500), CD45 (HI30, 1:500), and CD20 (HIB19, 1:500). Cells were also stained with DAPI (2 ng/ml) to exclude dead cells. Compensation was performed with OneComp eBeads beads (Thermo Fisher Scientific) or cells as appropriate. Samples were analyzed with a BD LSR II running DiVa software (BD Bioscience) and analyzed with FlowJo software (version 10.8). A minimum of 100 000 cells were employed for each analysis.
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