MRI evaluations were conducted on MPS IIIB and unaffected littermate dogs from Table 1 over six sessions, starting at approximately 8 months of age (session 1) and ending at 24 months of age (session 6, Supplemental Fig. 1A ). The intervening sessions occurred approximately every 3 months. MRI acquisition (three-dimensional, high-resolution, T1-weighted magnetization-prepared rapid gradient-echo pulse sequence) was performed on a 3 Tesla Siemens Prisma system with Tx/Rx 15-channel knee coil at the University of Minnesota, Twin Cities. Logistical constraints prevented collection of data from some animals and limited data collection on other animals. Volumes assessed included total brain, cerebral white matter, cerebral gray matter, ventricles, brainstem, cerebellar white matter, and cerebellar gray matter volumes. Total cerebellum and brainstem were manually outlined following the canine atlas (Singer 1962 ). After brain extraction using FSL brain extraction tool (Jenkinson et al., 2012 (link)) and manual correction of brain extraction tool inaccuracies, cerebral and cerebellar subvolumes were derived using FSL FAST (FMRIB’s Automated Segmentation Tool) (Zhang et al., 2001 (link)). All MRI data were collected and analyzed by individuals blinded to the genotype and treatment status of the dogs.
Tx rx 15 channel knee coil
Manufactured by Siemens
The Tx/Rx 15-Channel Knee Coil is a radio frequency (RF) coil used for magnetic resonance imaging (MRI) of the knee joint. It is designed to transmit and receive the RF signals required for MRI image acquisition. The coil features 15 independent channels to enhance signal-to-noise ratio and image quality.
Automatically generated - may contain errors
Lab products found in correlation
3 protocols using tx rx 15 channel knee coil
1
Longitudinal MRI Assessment of Canine MPS IIIB
2
MRI Evaluation of Tibial Osteotomy
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Dogs were placed in dorsal recumbency, with the affected leg placed in a knee coil (Siemens Tx/Rx 15-Channel Knee Coil) with the stifle flexed to about 120°. Images were obtained using a 3T MRI unit (Siemens Magnetom Skyra 3T, Erlangen, Germany) and using the parameters specified in Table 2 (Fig 6 ). Images were subsequently evaluated using Osirix version 6.5.2 (Bernex, Switzerland). The sagittal plane intermediate-weighted (IW) Dixon FS and dorsal plane T1-weighted (T1w) sequences were used simultaneously for region of interest (ROI) placement and to ensure no cortical or soft tissue partial volume averaging occurred within the ROI. Square ROIs (75mm2 area) were drawn on the sagittal plane images spanning the osteotomy (Fig 6 ). Values for the mean pixel intensity within the ROI were recorded. This process was repeated on 3 sagittal plane images that corresponded to the most medial, central, and most lateral portions of the proximal tibial medullary bone. Care was taken to ensure the most medial slice was not affected by perceptible metal artifact. The ROI evaluation of the T1w sagittal plane images was performed after the IW images using the same methods.
3
Knee MRI Acquisition Protocol for Osteoarthritis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
At baseline and 48-month follow-up, all participants were scanned with a 3 T MRI scanner (MAGNETOM Prisma; Siemens Healthcare) using a dedicated 15-channel knee coil (Tx/Rx 15-Channel Knee Coil, Siemens Healthcare). At both timepoints, a noncontrast isotropic fat-suppressed T2-weighted three-dimensional SPACE sequence was acquired for each knee. The slice position was aimed at the center of the femorotibial joint. Scan parameters: repetition time 1000 ms, echo time 108 ms, parallel imaging acceleration factor 4, acquired slice thickness 0.63 mm; field of view 160 × 160; matrix 256 × 256; receiver bandwidth, 415 Hz per pixel, leading to an acquisition time of 4 min 42 s [17 (link)].
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!