Solution of lipase B from Candida antarctica (CALB) (6.9 mg of protein/mL) was a kind gift from Novozymes (Alcobendas, Spain). Polyethylenimine (PEI) (MW 25,000), Triton X-100 and p-nitrophenyl butyrate (p-NPB) were purchased from Sigma-Aldrich (St. Louis, MI, USA). Octyl Sepharose CL-4B beads was from GE Healthcare Bio-Sciences (Uppsala, Sweden). Electrophoresis purity reagents were obtained from Bio-Rad (Hercules, CA, USA). All other reagents were of analytical grade. Protein concentration was estimated by the Bradford dye binding method at 595 nm [55 (link)] using bovine serum albumin as a standard.
Electrophoresis purity reagents
Manufactured by Bio-Rad
Sourced in United States
Electrophoresis purity reagents are a set of chemicals used in the process of electrophoresis, which is a technique used to separate and analyze biomolecules such as proteins, nucleic acids, and other macromolecules. These reagents are designed to ensure the purity and integrity of the samples being analyzed during the electrophoresis process.
Automatically generated - may contain errors
2 protocols using electrophoresis purity reagents
1
Lipase B Characterization from Candida antarctica
2
Immobilization of Candida antarctica and Rhizomucor miehie lipases
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Commercial soluble lipases from Candida antarctica (isoform B) and Rhizomucor miehie were kindly donated by Novozymes (Alcobendas, Spain). Octyl-agarose CL-4B beads were from GE Healthcare (Uppsala, Sweden). Polyethylenimine (Mw 25,000 Da), dextran sulfate (average Mw 20,000 Da), p-nitrophenyl butyrate (p-NPB) and 2,4,6-trinitrobenzenesulfonic acid solution (TNBS, 1 M in water) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Bradford protein assay kit and electrophoresis purity reagents were obtained from Bio-Rad (Hercules, CA, USA). Other reagents were of analytical grade.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!