White chicken feathers were supplied by a local poultry slaughterhouse (Drobful, Poland). NaOH (POCH, Poland) was used to carry out the hydrolysis. DTT and Coomassie brilliant blue R-250 (Fluka, USA), SDS (Merck, Germany), 30% solution of acyrlamide/bis-acrylamide, ammonium persulfate, bromophenol solution, glycine, hydrochloric acid, TEMED, and trizma base (Sigma-Aldrich, USA), acetic acid, glycerol and methanol (Chempur, Poland), ammonium perfulfate, and prestained protein ladder PageRuler Plus (Thermo scientific, USA) were applied for determination of protein molecular weight. Sulfuric acid and Tashiro’s indicator (POCH, Poland), selenium catalyst (Chempur, Poland), boric acid, and hydrochloric acid (Stanlab, Poland) were used to determine the protein content by the Kjeldahl method.
Solution of acyrlamide bis acrylamide
Manufactured by Merck Group
Sourced in Germany
30% solution of acrylamide/bis-acrylamide. This solution is a laboratory reagent commonly used in electrophoresis and other biochemical applications.
Automatically generated - may contain errors
Lab products found in correlation
Acetic acid, by Chempur (2 mentions)
Hydrochloric acid (hcl), by EKF Diagnostics (2 mentions)
Bromophenol solution, by Merck Group (2 mentions)
Glycerol and methanol, by Chempur (2 mentions)
Selenium catalyst, by Chempur (2 mentions)
Boric acid, by EKF Diagnostics (2 mentions)
Ammonium persulfate, by Merck Group (2 mentions)
Hydrochloric acid (hcl), by Merck Group (2 mentions)
Sodium dodecyl sulfate (sds), by Merck Group (2 mentions)
Pageruler plus, by Thermo Fisher Scientific (1 mentions)
2 protocols using solution of acyrlamide bis acrylamide
1
Feather Protein Extraction and Analysis
2
Chicken Feather Protein Extraction
Check if the same lab product or an alternative is used in the 5 most similar protocols
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White chicken feathers were supplied by a local poultry slaughterhouse (Drobful, Poland). NaOH (POCH, Poland) was used to carry out the hydrolysis. DTT and commasie brilliant blue R-250 (Fluka, USA), SDS (Merck, Germany), 30% solution of acyrlamide/bis-acrylamide, ammonium persulfate, bromophenol solution, glycine, hydrochloric acid, TEMED, and trizma base (Sigma-Aldrich, USA), acetic acid, glycerol and methanol (Chempur, Poland), ammonium perfulfate and prestained protein ladder PageRuler Plus (Thermo scienti c, USA) were applied for determination of protein moleculat weight. Sulfuric acid and Tashiro's indicator (POCH, Poland), selenium catalyst (Chempur, Poland), boric acid and hydrochloric acid (Stanlab, Poland) were used to determine the protein content by the Kjeldahl method.
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