Nvista acquisition software

Manufactured by Inscopix

Sourced in United States

NVista is a software suite designed for the acquisition and control of Inscopix's NVista in vivo imaging systems. The software enables data collection from these imaging platforms, providing users with the necessary tools to capture and manage their experimental data.

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Lab products found in correlation

Baseplate, by Inscopix (1 mentions) Grin lens, by Inscopix (1 mentions) Nvista, by Inscopix (1 mentions) Io box system, by Noldus (1 mentions)

5 protocols using nvista acquisition software

Calcium Imaging in Freely Moving Mice

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Calcium imaging was performed in freely moving mice in a Y-maze using the head-attached microscope (Inscopix; 2 mm diameter objective lens; LED power: 0.6 – 1.0 mW; camera resolution: 1440 x1080 pixels). Images were acquired at 30 Hz using nVista Acquisition Software (Inscopix). At the beginning of each imaging session, the protective baseplate cover of the previously implanted baseplate was removed and the microscope was attached in its place. The imaging field of view was approximately 900 × 600 µm² at 0.65 µm/pixel resolution and the imaging depth was selected by adjusting the focus of the microscope until clear cell signals were observed in the online ΔF/F images.

Li X., Cao V.Y., Zhang W., Mastwal S.S., Liu Q., Otte S, & Wang K.H. (2017). Skin suturing and cortical surface viral infusion improves imaging of neuronal ensemble activity with head-mounted miniature microscopes. Journal of neuroscience methods, 291, 238-248.

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In-vivo imaging of AI neuron activity

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Activity of GCaMP6f-labeled AI neurons during social behavior tests was imaged using a miniaturized head-mounted microscope and GRIN lens-mediated microendoscopy (Fig 2A). Before the experiments, mice were lightly anesthetized with 2% isoflurane, and the baseplate cover was removed from the baseplate, to which a miniaturized head-mounted microscope was subsequently attached. Mice were then recovered from anesthesia for at least 20 min before beginning the experiments.
Ca²⁺ imaging videos were recorded during social behavior tests using nVista acquisition software (version 1.2.0; Inscopix, Palo Alto, CA) with a resolution of 1,440 × 1,080 pixels at a rate of 15 frames/s. The LED power of the microscope was set between 30% and 50% (0.36–0.6 mW). Ca²⁺ transients were observed in many neurons within the FOV while mice moved freely in their environments (Fig 2B, 81.9 ± 44.6 cells/FOV, mean ± SD, n = 9 mice), and post hoc confirmation of the lens positions verified that GRIN lenses were successfully targeted to the AI in all 9 cases (Fig 2C).

Miura I., Sato M., Overton E.T., Kunori N., Nakai J., Kawamata T., Nakai N, & Takumi T. (2020). Encoding of social exploration by neural ensembles in the insular cortex. PLoS Biology, 18(9), e3000584.

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In vivo Ca2+ Imaging of Fear Memory Circuits

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Ca²⁺ imaging of PrL and BLA neurons was performed on WT mice. AAV₅-hsyn-GCaMP6f (Tailtool, S02245) was injected into right PrL (AP: 1.94 mm, ML: +0.5 mm, DV: −2.15 mm) or BLA (AP: −1.46 mm, ML: +3.3 mm, DV: −4.68 mm), then a GRIN lens (0.5-mm diameter, 4.1-mm length; Inscopix) was implanted on PrL, and a GRIN lens (0.5-mm diameter, 6.1-mm length; Inscopix) was implanted on BLA after 2-week injection. Last, a baseplate (Inscopix) was attached above the GRIN lens by ultraviolet-light curable glue 2 weeks after GRIN lens implantation. The Ca²⁺ imaging data were captured (20 frames/s) using the Inscopix miniature microscope and nVista acquisition software (Inscopix, CA, USA) during retrieval, extinction, EM test, and SR test. In all the fear memory–related behavior experiments, a Transistor-Transistor-Logic (TTL) signal was used to synchronize the calcium signal and the behavioral time points.

Teng S.W., Wang X.R., Du B.W., Chen X.L., Fu G.Z., Liu Y.F., Xu S.Q., Shuai J.C, & Chen Z.Y. (2023). Altered fear engram encoding underlying conditioned versus unconditioned stimulus–initiated memory updating. Science Advances, 9(23), eadf0284.

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In Vivo Calcium Imaging of Neuronal Activity

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Six days or more after setting the baseplate, mice were habituated to the experimental room for at least 1 day. Mice were lightly anesthetized with isoflurane to mount the miniature microscope. When the mice became quiet (resting state) at least 30 min after their recovery from the anesthesia, Ca²⁺ imaging was performed while the mice were in their home cage (habituation session). The imaging data were acquired for 10 min, and the microscope was detached after an additional 10 min. On the following day, Ca²⁺ imaging was similarly performed in the home cage (pre session) followed by Ca²⁺ imaging for 10 min in a novel square context (Muromachi Kikai, Co., Ltd., Japan; sq. session). The mice were then returned to their home cage (see Fig. 1b).
The square context had a Plexiglass front, gray sides, and back walls (width × depth × height: 175 mm × 165 mm × 400 mm), and the chamber floors comprised 26 stainless steel rods (2 mm diameter) placed 5 mm apart.
Calcium imaging was performed during the light cycle. Imaging data were acquired using nVista acquisition software (Inscopix, Inc.) at 1440 × 1280 pixels, at 20 frames/s, maximum gain and at optimal LED power (based on the histogram, according to the nVista user guide).

Asai H., Ohkawa N., Saitoh Y., Ghandour K., Murayama E., Nishizono H., Matsuo M., Hirayama T., Kaneko R., Muramatsu S.I., Yagi T, & Inokuchi K. (2020). Pcdhβ deficiency affects hippocampal CA1 ensemble activity and contextual fear discrimination. Molecular Brain, 13, 7.

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In Vivo Calcium Imaging and Behavioral Synchronization

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Awake-behaving imaging sessions were commenced approximately 1 week after baseplating. On each imaging day, mice were briefly anesthetized (< 5 minutes) with 3% isoflurane in order to attach the miniscope to the baseplate. Mice were allowed to recover from anesthesia in their home cage for 20 minutes before imaging. Ca²⁺ videos were recorded using nVista acquisition software (Inscopix, Palo Alto, CA), and triggered with a TTL pulse from EthoVision XT 11 via the Noldus IO box system to allow for simultaneous acquisition of Ca²⁺ and behavioral videos. To later account for any lag between the onset of behavior and Ca²⁺ movies, a continuous train of TTL pulses was sent from Ethovision XT 11 to nVista acquisition software at 0.5 Hz and a 50% duty cycle for the duration of the session for running synchronization of the two datasets.
Ca²⁺ videos were acquired at 17 frames per second with an automatic exposure length. An optimal LED power was selected for each mouse to optimize the dynamic range of pixel values in the field of view, and the same LED settings were used for each mouse throughout the series of imaging sessions. The maximum LED power used was 85% and movies were collected without gain.

Paquelet G.E., Carrión K., Lacefield C.O., Zhou P., Hen R, & Miller B.R. (2022). Single-cell activity and network properties of dorsal raphe nucleus serotonin neurons during emotionally salient behaviors. Neuron, 110(16), 2664-2679.e8.

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