Polyu and polya rna

Manufactured by Merck Group

PolyU and PolyA RNA are types of synthetic RNA molecules used in various laboratory applications. PolyU refers to a polynucleotide chain composed solely of uridine (U) nucleotides, while PolyA refers to a polynucleotide chain composed solely of adenosine (A) nucleotides. These RNA molecules are utilized as tools in molecular biology and biochemistry research, but their specific applications and intended uses are not provided in this factual description.

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Lab products found in correlation

Bis ans, by Thermo Fisher Scientific (2 mentions) Potassium chloride (kcl), by Thermo Fisher Scientific (2 mentions) Polyu100 rna, by Biomers (2 mentions) Alexa647 carboxylic acid, by Thermo Fisher Scientific (2 mentions) Peg 4000 and 6000, by Merck Group (2 mentions) Alexa 546, by Thermo Fisher Scientific (2 mentions) Dimethyl sulfoxide (dmso), by Merck Group (2 mentions)

2 protocols using polyu and polya rna

Characterization of SARS-CoV-2 Nucleocapsid Protein Interactions

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All reagents and chemicals were purchased with the highest purity available. Tris as a buffer reagent and KCl were from Fisher Scientific. PolyU and PolyA RNA with a molecular weight range from 800–1000 kDa was purchased from Sigma Aldrich as lyophilized powder. PolyU₁₀₀ RNA was purchased from Biomers as lyophilized power and dissolved into a stock of 1 mg/mL in mQ water before use 1,6-HD was purchased from Santa Cruz Biotechnology. Bis-ANS was purchased from Invitrogen and dissolved to make a stock solution in DMSO (Sigma Aldrich). Alexa546 and Alexa647 carboxylic acid were obtained from Thermo Fisher. PEG 4000 and 6000 were from Sigma Aldrich. Details on the production of proteins (FUS^G156E-EGFP, G3BP1-GFP, NSP2/5, SARS-CoV-2 N and (PR)₂₅ are given in the Supplementary Information.

Arter W.E., Qi R., Erkamp N.A., Krainer G., Didi K., Welsh T.J., Acker J., Nixon-Abell J., Qamar S., Guillén-Boixet J., Franzmann T.M., Kuster D., Hyman A.A., Borodavka A., George-Hyslop P.S., Alberti S, & Knowles T.P. (2022). Biomolecular condensate phase diagrams with a combinatorial microdroplet platform. Nature Communications, 13, 7845.

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Biomolecular Interactions in Stress Granules

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All reagents and chemicals were purchased with the highest purity available. Tris as a buffer reagent and KCl were from Fisher Scientific. PolyU and PolyA RNA with a molecular weight range from 800-1000 kDa was purchased from Sigma Aldrich as lyophilized powder. PolyU100 RNA was purchased from Biomers as lyophilized power and dissolved into a stock of 1 mg/mL in mQ water before use 1,6-HD was purchased from Santa Cruz Biotechnology. Bis-ANS was purchased from Invitrogen and dissolved to make a stock solution in DMSO (Sigma Aldrich).
Alexa546 and Alexa647 carboxylic acid were obtained from Thermo Fisher. PEG 4000 and 6000 were from Sigma Aldrich. Details on the production of proteins (FUS G156E -EGFP, G3BP1-GFP, NSP2/5, SARS-CoV-2 N and (PR)25 are given in the Supporting Information.

Arter W.E., Qi R., Erkamp N.A., Krainer G., Didi K., Welsh T.J., Acker J., Nixon‐Abell J., Qamar S., Guillén‐Boixet J., Franzmann T.M., Kuster D., Hyman A., Borodavka A., George‐Hyslop P.S., Alberti S., & Knowles T.P.J. (2020). High Resolution Biomolecular Condensate Phase Diagrams with a Combinatorial Microdroplet Platform.

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