Alpha smooth muscle actin clone 1a4

Cases were identified in the consultation files of the authors. The tissue specimens were fixed in formalin and processed routinely for histopathology. Immunohistochemistry (IHC) was performed on 3-μm sections cut from paraffin blocks using a fully automated system (“Benchmark XT System”, Ventana Medical Systems Inc., 1910 Innovation Park Drive, Tucson, Arizona, USA) and the following antibodies: vimentin (V9, 1:100, Dako), keratin cocktail (clone AE1/AE3, 1:40, Zytomed, Berlin, Germany), p63 (SSI6, 1: 100, DCS), desmin (clone D33, 1:250, Dako), alpha smooth muscle actin (clone 1A4, 1:200, Dako), HMB45 (clone HMB45, 1:50, Enzo), Melan A (clone A103, 1:50, Dako), CD34 (clone BI-3C5, 1:200, Zytomed), ERG (EPR3864, prediluted, Ventana), CD31 (clone JC70A, 1:20, Dako), S100 protein (polyclonal, 1:2500, Dako), SOX10 (polyclonal, 1:25, DCS), PAX8 (polyclonal rabbit anti-PAX8, 1:50, Cell Marque), NSE (clone BBS/NC/VI-H1, 1:300, Dako), TTF1 (clone 8G7G3/1, 1:500, Zytomed Systems, Berlin, Germany), Napsin A (MRQ-60, ready-to-use, Medac), calretinin (polyclonal, 1:100, Zytomed), alpha-inhibin (clone R1, 1:50, Serotec), GFAP (Clone GFA, 1/1000, DakoPatts, Denmark), EMA (clone E29, 1:200, Dako), STAT6 (clone S-20, 1:1000, Santa Cruz Biotechnology), TFE3 (clone MRQ-37, 1:100, Cell Marque), Cathepsin-K (clone 3F9, 1:50, Abcam) and Ki67 (clone MiB1, 1:100, Dako).