Standard methods were used to prepare and visualize bacterial samples [26 ]. Briefly, bacteria were grown at 28 °C for 24–36 h in BHI, centrifuged at 6000×g for 10 m, and resuspended to 0.5 McFarland in DPBS. 1 mL of resuspension was bound to lyophilized CN3080 Nanotraps following standard unlysed bacteria binding protocol, with variations of bacterial concentrations. Samples were fixed for 30 min with a 5% glutaraldehyde solution in 0.1 M phosphate buffer (pH 7.2). Fixative was discarded and the Nanotrap pellet was washed twice with 0.1 M phosphate buffer. Following carbon coating, fixed cells and Nanotraps were visualized using the Hitachi SU-70 Schottky field emission gun scanning electron microscope at University of Maryland’s Advanced Imaging & Microscopy Laboratory (College Park, MD).
Su 70 schottky field emission gun scanning electron microscope
Manufactured by Hitachi
The SU-70 Schottky field emission gun scanning electron microscope is a high-performance laboratory instrument designed for advanced materials analysis. It utilizes a Schottky field emission gun to produce a finely focused electron beam, enabling high-resolution imaging and detailed elemental analysis of a wide range of samples.
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Lab products found in correlation
F 7000 fluorescence spectrophotometer, by Hitachi (1 mentions)
Sz 100, by Horiba (1 mentions)
Tecnai tf30 transmission electron microscope, by Thermo Fisher Scientific (1 mentions)
Ultrascan 1000 ccd camera, by Ametek (1 mentions)
Genesys 10s uv vis spectrophotometer, by Thermo Fisher Scientific (1 mentions)
La 950 laser diffraction analyzer, by Horiba (1 mentions)
5 protocols using su 70 schottky field emission gun scanning electron microscope
1
Bacterial Visualization Using Nanotraps
2
Erythrocyte Toxicity Evaluation of CPT&siR CRNPs
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Whole blood collected from C57BL/6 mice was centrifuged at 500 g and washed with PBS for 3 times. A total of 0.5 ml of whole blood cells (4% in PBS) were mixed with 0.5 ml of DW, PBS, or CPT&siR CRNPs at various CRNP concentrations from 25 to 800 µg ml−1. After incubation for 24 h at 37 °C, the samples were centrifuged at 500 g for 5 min. Supernatants were collected and their absorbances at 540 nm were measured by the Spark Multimode Microplate Reader. In addition, after dehydration by a series of ethanol solutions (50%, 75%, 85%, 95%, and 100%), the morphology of erythrocytes after incubation with CPT&siR CRNPs at 800 µg ml−1 was compared to that from the PBS group via taking their SEM images using a Hitachi SU-70 Schottky field emission gun scanning electron microscope by following the procedure provided by the manufacturer.
3
Nanofiber Morphology and Composition Analysis
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A Hitachi SU‐70 Schottky field emission gun scanning electron microscope was used to image nanofiber mats sputter coated with gold. Snapshots were taken across the surface of the fiber mat. Fiber diameter and porosity were determined using the DiameterJ plugin for ImageJ (n = 2–4).64 EDS was used to measure weight fraction. EDS‐determined abundances were converted to weight fraction based on the three primary elemental components of the polymer fibers being carbon, oxygen, and silver.
4
Comprehensive Material Characterization Techniques
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Fluorescence spectra were recorded by using a Hitachi F-7000 fluorescence spectrophotometer. The UV-Vis absorption spectra were recorded via a Genesys 10s UV-Vis spectrophotometer (Thermo Scientific). Dynamic light scattering (DLS) measurement was carried out on a scientific nanoparticle analyzer (SZ-100, Horiba). Powder X-ray diffraction (XRD) patterns were observed on a Rigaku Dmax2550PC polycrystal X-ray diffractometer using Cu Kα radiation (Rigaku Co., Tokyo, Japan). Transmission electron microscopy (TEM) images were recorded on a Tecnai TF30 transmission electron microscope (FEI, Hillsboro, OR) equipped with a Gatan Ultrascan 1000 CCD camera (Gatan, Pleasaton, CA). Scanning electron microscope (SEM) imaging was performed on a Hitachi SU-70 Schottky field emission gun scanning electron microscope.
5
Characterization of PolyIC-loaded Microparticles
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