Research blood samples collected from enrolled participants into yellow-top Vacutainer tubes were subsequently inoculated into Bactec Plus Aerobic/F blood culture bottles (BD# 442192) upon receipt in the research lab and immediately incubated at 37°C with continuous motion (150 rpm, Excella E24 Incubator Shaker Series, New Brunswick Scientific, Enfield, CT). Samples were rejected for this study if the blood in the vacutainer tube was grossly hemolyzed, clotted, or contained half or less of the fill blood volume. Time of inoculation was noted for each culture bottle, with 1.5 ml aliquots of culture fluid removed aseptically from each aerobic blood culture bottle after 8 h for this analysis. Research specimens were inoculated only into aerobic blood culture bottles, as this study was not intended to evaluate strict anaerobic organisms.
Excella e24 incubator shaker series
Manufactured by Eppendorf
Sourced in United States
The Excella E24 Incubator Shaker Series is a laboratory equipment designed for controlled incubation and shaking. It provides a temperature-controlled environment for cell culture, microbiological, and biochemical applications.
Automatically generated - may contain errors
Lab products found in correlation
4 protocols using excella e24 incubator shaker series
1
Blood Culture Sample Processing
2
Dual-Species Biofilm Formation and Treatment
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Acrylic discs were incubated with fetal bovine serum (FBS) (Gibco; Life Technologies, Carlsbad, CA, USA) in a shaker incubator (Excella E24 Incubator Shaker Series; New Brunswick Scientific, Enfield, CT, USA) at 75 rpm and 37 °C for 24 h. Then, specimens were washed once with 0.1 M PBS, and placed in 24-well plate. Dual-species biofilms were developed by adding 500 µL of each cell suspension in a well of 24-well plate. The plates were incubated aerobically in a shaker incubator at 75 rpm and 37 °C for 24 h.
After dual-species biofilm growth for 24 h, each biofilm sample was gently washed with 1000 µL of 0.1 M PBS twice for 10 s each time to remove any non-adherent cells. The samples without further exposed to test agents were used as the untreated control. For the other groups, biofilm samples were immersed in each test agent as mentioned above for 3 and 15 min, except 3 min-Compound 1 group was treated by spraying according to a previous study22 (link). Following exposure to test agents, residual biofilm samples were washed twice with 1000 µL of 0.1 M PBS.
After dual-species biofilm growth for 24 h, each biofilm sample was gently washed with 1000 µL of 0.1 M PBS twice for 10 s each time to remove any non-adherent cells. The samples without further exposed to test agents were used as the untreated control. For the other groups, biofilm samples were immersed in each test agent as mentioned above for 3 and 15 min, except 3 min-Compound 1 group was treated by spraying according to a previous study22 (link). Following exposure to test agents, residual biofilm samples were washed twice with 1000 µL of 0.1 M PBS.
3
Cultivation of Mucor circinelloides Strain
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The fungal strain Mucor circinelloides f. lusitanicus (ATCC® 1216B™) was obtained from the American Type Culture Collection (Manassas, VA, USA). Spore suspensions were prepared as described previously in potato dextrose broth and glycerol and stored at − 80 °C [16 (link)]. The spore suspension was used as the inoculum. A 1% M. circinellodes preculture (~ 1 mL inoculum per 100 mL of media) was prepared in a 250 mL baffled Erlenmeyer shake flask containing potato dextrose broth. The flask was placed on a rotary shaker (Excella E24 Incubator Shaker Series, New Brunswick Scientific, New York) at 34 °C with an agitation speed of 200 rpm for 24 h.
4
Sulfur-limited Bacterial Growth Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
E. coli MG1655 and P. fluorescens WCS365 were grown in batch cultures on glucose in 1 L M9 minimal media that was modified to use ammonium sulfate as the sole sulfur source. The recipe was as follows: in 1L add 7.52 g Na2HPO4 • 2H2O, 3.0 g KH2PO4, 0.5 g NaCl, 2.5 g (NH4)2SO4, 1 mL 0.1 M CaCl2, 1 mL 1.0 M MgCl2, 4 g glucose, and 1 mL 1000x vitamins mix (DSM141 recipe). Initial inoculation occurred in 10-mL culture tubes before transfer to a 1 L Erlenmeyer flask. Cultures were kept at 37 ˚C on an Excella E24 Incubator Shaker Series (New Brunswick Scientific, Edison, NJ, USA) and grown overnight at 250 RPM. Cell growth was monitored by measuring OD600 on a DU 800 UV/VIS spectrophotometer (Beckman Coulter, Brea, CA, USA). Cells were harvested in mid-log phase, at OD600 ~1, and washed twice with 0.9% NaCl at 4 °C. Pellets were stored at -20 ˚C until analysis.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!