Proxpress visible uv fluorescence 16 bit scanner

Manufactured by PerkinElmer

The ProXpress Visible-UV-fluorescence 16-bit scanner is a multi-purpose imaging device designed for high-performance capture of visible, ultraviolet, and fluorescent samples. It features a 16-bit color depth, enabling precise and detailed image acquisition.

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Lab products found in correlation

Celltiter blue, by Promega (4 mentions) Envision multilabel reader, by PerkinElmer (2 mentions) Celltiter glo 2.0 luminescence assay kit, by Promega (2 mentions) Wst 1 reagent, by Merck Group (1 mentions)

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ProXPRESS (3 citations) Fluorescence scanner (2 citations)

4 protocols using proxpress visible uv fluorescence 16 bit scanner

NSCLC Cell Line Viability Assay

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The A549 and H441 human NSCLC cell lines were obtained from the American Type Culture Collection (ATCC). Cells (1 × 103 cells/well) were plated in RPMI1640 (H441) or DMEM (A549) media with 10% FBS in 96-well cell culture plates. Vehicle (0.1% DMSO), STA-8666 or irinotecan were added in concentrations of 0.1, 1 and 10 μM to each well, and cells were grown for up to 5 days, in duplicate for each of 5 assay time points. On days 1, 2, 3, 4, or 5 after plating, CellTiter-Blue^® (Promega, Fitchburg, WI) reagent was added to all wells; after 4 hours incubation at 37°C, optical density readings were made in the 570–600 and 420–480 nm wave-length ranges, respectively, using Perkin-Elmer ProXpress Visible-UV-fluorescence 16 bit scanner (Perkin-Elmer, Waltham, MA). Values from at least two independent experiments were averaged.

Deneka A.Y., Haber L., Kopp M.C., Gaponova A.V., Nikonova A.S, & Golemis E.A. (2017). Tumor-targeted SN38 inhibits growth of early stage non-small cell lung cancer (NSCLC) in a KRas/p53 transgenic mouse model. PLoS ONE, 12(4), e0176747.

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Cell Viability Assays for Drug Testing

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Cells were plated in 24 or 96 well plates and cultured for 24 hours. Dinaciclib, CFT-2718 or vehicle were diluted in cell culture medium and added to plates. For some assays, after 72 hours, CellTiter-Blue® (Promega, Fitchburg, WI) reagent was added to each well. After two hours incubation at 37°C, optical density readings were made in the 570 – 600 nm wave-length range, using a Perkin-Elmer ProXpress Visible-UV-fluorescence 16-bit scanner (Perkin-Elmer, Waltham, MA). In other experiments, a CellTiter-Glo® 2.0 luminescence Assay kit (Promega, Madison, WI) was used as an alternative means to assess viability, with data acquired on an EnVision^™ Multilabel Reader (PerkinElmer, Santa Clara, CA, USA). In these assays, a time zero plate was incorporated into the assay to define GI50, TGI and LC50 (36 ).

Sun D., Nikonova A.S., Zhang P., Deneka A.Y., Fitzgerald M.E., Michael R.E., Lee L., Lilly A.C., Fisher S.L., Phillips A.J., Nasveschuk C.G., Proia D.A., Tu Z, & Golemis E.A. (2021). Evaluation of the Small-molecule BRD4 Degrader CFT-2718 in Small-cell Lung Cancer and Pancreatic Cancer Models. Molecular Cancer Therapeutics, 20(8), 1367-1377.

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Cell Viability Assay in 96-well Plates

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Cells (1–2 × 10³/well) were plated in quadruplicate plates in RPMI1640 media with 10% FBS in 96-well cell culture plates for 5 days. On days 1–5, CellTiter-Blue^® (Promega, Fitchburg, WI) or WST-1 reagent (Sigma-Aldrich, St. Louis, MO) reagent were added to wells one one plate; after 2 hours incubation at 37°C, optical density readings were made to visualize percentage of live cells, using a Perkin-Elmer ProXpress Visible-UV-fluorescence 16-bit scanner (Perkin-Elmer, Waltham, MA). GraphPad software used for statistical analysis.

Kharin L., Bychkov I., Karnaukhov N., Voloshin M., Fazliyeva R., Deneka A., Frantsiyants E., Kit O., Golemis E, & Boumber Y. (2021). Prognostic role and biologic features of Musashi-2 expression in colon polyps and during colorectal cancer progression. PLoS ONE, 16(7), e0252132.

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Cell Viability Assay for Drug Evaluation

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Cells were plated in 24- or 96-well plates and cultured for 24 hours. Dinaciclib, CFT-2718, or vehicle were diluted in cell culture medium and added to plates. For some assays, after 72 hours, CellTiter-Blue (Promega) reagent was added to each well. After 2-hour incubation at 37°C, optical density readings were made in the 570- to 600-nm wavelength range, using a Perkin-Elmer ProXpress Visible-UV-fluorescence 16-bit scanner (Perkin-Elmer). In other experiments, a CellTiter-Glo 2.0 luminescence Assay Kit (Promega) was used as an alternative means to assess viability, with data acquired on an EnVision Multilabel Reader (PerkinElmer). In these assays, a time zero plate was incorporated into the assay to define GI₅₀, TGI, and LC₅₀ (36 ).

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