Most cell lines were originally purchased from the American Type Culture Collection (ATCC) and were cultured as per the standard ATCC protocols. LNCaP-AR and LAPC4 cells were gifts from Dr. Charles Sawyers lab (Memorial Sloan-Kettering Cancer Center, New York, NY). Until otherwise stated, for all the experiments LNCaP, PNT2, LNCaP-AR, C42B, 22RV1, DU145, PC3 cells were grown in the RPMI 1640 medium (Gibco) and VCaP cells in the DMEM with Glutamax (Gibco) medium supplemented with 10% Full Bovine Serum (FBS; Invitrogen). LAPC4 cells were grown in IMEM (Gibco) medium supplemented with 15%FBS and 1nM of R1881. Immortalized normal prostate cells: RWPE1 were grown in keratinocyte media with regular supplements (Lonza); PNT2 were grown in RPMI medium with 10%FBS. HEK293 cells were grown in DMEM (Gibco) medium with 10% FBS. All cells were grown in a humidified 5%CO2 incubator at 37℃. All cell lines were biweekly tested to be free of mycoplasma contamination and genotyped every month at the University of Michigan Sequencing Core using Profiler Plus (Applied Biosystems) and compared with corresponding short tandem repeat (STR) profiles in the ATCC database to authenticate their identity in culture between passages and experiments.
Rwpe1
Manufactured by Lonza
Sourced in Switzerland
RWPE1 is a cell line derived from normal human prostate epithelial cells. It serves as a cell model for research purposes.
Automatically generated - may contain errors
Lab products found in correlation
Regular supplements, by Lonza (2 mentions)
Full bovine serum fbs, by Thermo Fisher Scientific (2 mentions)
Lapc4, by Thermo Fisher Scientific (2 mentions)
Profiler plus, by Thermo Fisher Scientific (2 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (2 mentions)
Glutamax, by Thermo Fisher Scientific (2 mentions)
Rpmi 1640 medium, by Thermo Fisher Scientific (2 mentions)
Penicillin, by GE Healthcare (1 mentions)
Streptomycin, by GE Healthcare (1 mentions)
F 12k medium, by Corning (1 mentions)
Du145, by Corning (1 mentions)
Dmem ham s f 12 medium, by Corning (1 mentions)
Kgm gold single quot kit, by Lonza (1 mentions)
Sv huc 1, by Corning (1 mentions)
Amphotericin b, by GE Healthcare (1 mentions)
Sv huc 1, by American Type Culture Collection (1 mentions)
Du145, by American Type Culture Collection (1 mentions)
Rwpe 1, by American Type Culture Collection (1 mentions)
Fetal bovine serum (fbs), by PAN Biotech (1 mentions)
3 protocols using rwpe1
1
Prostate Cancer Cell Line Cultivation
2
Prostate Cancer Cell Line Cultivation
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Most cell lines were originally purchased from the American Type Culture Collection (ATCC) and were cultured as per the standard ATCC protocols. LNCaP-AR and LAPC4 cells were gifts from Dr. Charles Sawyers lab (Memorial Sloan-Kettering Cancer Center, New York, NY). Until otherwise stated, for all the experiments LNCaP, PNT2, LNCaP-AR, C42B, 22RV1, DU145, PC3 cells were grown in the RPMI 1640 medium (Gibco) and VCaP cells in the DMEM with Glutamax (Gibco) medium supplemented with 10% Full Bovine Serum (FBS; Invitrogen). LAPC4 cells were grown in IMEM (Gibco) medium supplemented with 15%FBS and 1nM of R1881. Immortalized normal prostate cells: RWPE1 were grown in keratinocyte media with regular supplements (Lonza); PNT2 were grown in RPMI medium with 10%FBS. HEK293 cells were grown in DMEM (Gibco) medium with 10% FBS. All cells were grown in a humidified 5%CO2 incubator at 37℃. All cell lines were biweekly tested to be free of mycoplasma contamination and genotyped every month at the University of Michigan Sequencing Core using Profiler Plus (Applied Biosystems) and compared with corresponding short tandem repeat (STR) profiles in the ATCC database to authenticate their identity in culture between passages and experiments.
3
Cell Culture Conditions for Cancer Research
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SV-HUC-1 (non-malignant human urothelium), T24 (human bladder cancer), DU-145 (human prostate cancer), and RWPE-1 (non-malignant human prostate epithelium) cell lines were purchased from the American Type Culture Collection (Manassas, VA, USA). T24 and DU-145 were cultured in DMEM/Ham’s F-12 medium (Corning, New York, NY, USA) containing 10% fetal bovine serum (FBS, Pan-Biotech GmbH, Aidenbach, Germany) supplemented with 5 μg/mL of amphotericin B (GE Healthcare Life Science, Chicago, IL, USA), 100 μg/mL of streptomycin, and 100 U/mL of penicillin (GE Healthcare Life Science, Chicago, IL, USA). SV-HUC-1 was cultured in F-12K medium (Corning, New York, NY, USA) with the same supplementation. RWPE-1 was cultured in KBM-Gold™ Keratinocyte Cell Basic Medium (Lonza, Basel, Switzerland) supplemented with a KGM-Gold SingleQuot Kit (Lonza, Basel, Switzerland). All cell lines were grown in a plastic tissue culture 75 cm2 T-flasks (Corning, New York, NY, USA) at 37 °C and 5% CO2.
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