Pooled human cerebrospinalfluid

Manufactured by Innovative Research

Pooled human cerebrospinalfluid is a biological sample collected from multiple individuals. It consists of the clear fluid that circulates in the space surrounding the brain and spinal cord. This product is intended for research and laboratory applications.

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Lab products found in correlation

Human tnf α, by Thermo Fisher Scientific (1 mentions) Milli q system, by Merck Group (1 mentions) Bovine serum albumin (bsa), by Merck Group (1 mentions) Human serum, by Merck Group (1 mentions) Potassium ferricyanide, by Merck Group (1 mentions) Sulfo smcc, by Thermo Fisher Scientific (1 mentions) Ultrapure 1 m tris hcl, by Thermo Fisher Scientific (1 mentions) Li heparin, by Innovative Research (1 mentions) K3 edta, by Innovative Research (1 mentions) Nhs peg4 biotin, by Thermo Fisher Scientific (1 mentions) Na citrate, by Innovative Research (1 mentions) K2 edta, by Innovative Research (1 mentions) Pooled human plasma, by Innovative Research (1 mentions)

2 protocols using pooled human cerebrospinalfluid

Electrochemical Cytokine Detection Assay

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3-Mercaptopropionic acid, N-hydroxysuccinimide (NHS), N-(3-(dimethylamino)propyl)-N′-ethylcarbodiimide hydrochloride (EDC), sulfuric
acid (95–98% ACS Reagent), hydrogen peroxide 30% (w/w) solution,
ethanol, 2-(N-morpholino)ethanesulfonic acid (MES),
phosphate buffered saline (PBS, pH 7.4), monoclonal anti-interleukin-6
antibody, Tween 20, bovine serum albumin (lyophilized), potassium
ferricyanide, potassium ferrocyanide, lithium perchlorate, human interleukin-6
(recombinant, expressed in E. coli, lyophilized),
human interleukin-10 (recombinant, expressed in E. coli, lyophilized), and human serum (heat-inactivated, clotted from whole
blood) were purchased from Millipore Sigma. IgG H&L Alexa Fluor
568 (Abcam), human TNF-α (recombinant, expressed in E. coli, lyophilized, PeproTech), pooled human cerebrospinal
fluid (Innovative Research), and Au interdigitated electrodes on glass
(10 μm digit/gap, Metrohm Dropsens) were also used. Aqueous
solutions were prepared using deionized (DI) water (ρ = 18.2
MΩ cm) from a Millipore Milli-Q system.

Oh C., Park B., Li C., Maldarelli C., Schaefer J.L., Datta-Chaudhuri T, & Bohn P.W. (2021). Electrochemical Immunosensing of Interleukin-6 in Human Cerebrospinal Fluid and Human Serum as an Early Biomarker for Traumatic Brain Injury. ACS Measurement Science Au, 1(2), 65-73.

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HIV-1 Immunoassay Reagents and Protocols

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The following matrixes were obtained from Innovative Research: Pooled Human Plasma (blood derived) with the anticoagulants K2 EDTA (cat# IPLAWBK2E), K3 EDTA (cat# IPLAWBK3E), Na EDTA (cat# IPLAWBNAE), Li Heparin (cat# IPLAWBLIH5), Na Heparin (cat# IPLAWBNAH), Na Citrate (cat# IPLAWBNAC), Pooled Human Cerebrospinal Fluid (cat# IRHUCSF), Pooled Human Pasteurized Breast Milk (cat# IRHUBMKPST) (pooled with a minimum of three donors), and Pooled Human AB Serum Plasma Derived (cat# ISERAB) (pooled from male donors blood type AB). Anti-HIV type 1 p24 clone 39/5.4A (cat# 0801136) was purchased from Zeptometrix. Monoclonal anti-HIV-1/2 purified antibody (cat# HIV-018-48303) was purchased from Capricorn Products LLC. The following reagents were obtained from Thermo Fisher Scientific: Sulfo-SMCC (cat# A39268), NHS-PEG₄-Biotin (cat# A39259) and Ultrapure 1 M Tris–HCl (cat# 15-567-027). Simoa Planar Array Homebrew Tag 1, Sample Diluent A, Streptavidin-HRP, Stable Peroxide, SuperSignal Luminol, Homebrew plate, and 25X wash buffer were all obtained from Quanterix. The following reagent was obtained through the NIH HIV Reagent Program, Division of AIDS, NIAID, NIH: Human Immunodeficiency Virus 1 (HIV-1), Strain JR-CSF Infectious Molecular Clone (pYK-JRCSF), ARP-2708, contributed by Dr. Irvin SY Chen and Dr. Yoshio Koyanagi.

Levinger C., Howard J.N., Cheng J., Tang P., Joshi A., Catalfamo M, & Bosque A. (2021). An ultrasensitive planar array p24 Gag ELISA to detect HIV-1 in diverse biological matrixes. Scientific Reports, 11, 23682.

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