Rea control antibody

Manufactured by Miltenyi Biotec

The REA Control Antibody is a laboratory reagent used to validate the performance of flow cytometry instruments and procedures. It provides a consistent, standardized sample for quality control and instrument calibration purposes.

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Lab products found in correlation

Cm3050s cryostat, by Leica (1 mentions) Evos m5000, by Thermo Fisher Scientific (1 mentions) Cd31 apc, by Miltenyi Biotec (1 mentions) Epcam fitc, by Miltenyi Biotec (1 mentions) Facsaria 2 flow cytometer, by BD (1 mentions) Lsrii flow cytometer, by BD (1 mentions) Facsdiva software, by BD (1 mentions) Aldefluor assay kit, by STEMCELL (1 mentions) Aldered aldh detection assay kit, by Merck Group (1 mentions)

Spelling variants of this product

REA control human IgG1 APC-conjugated isotype antibody (2 citations)

2 protocols using rea control antibody

Immunohistochemical Staining of Tissue Sections

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Tissue specimens were embedded into OCT mounting medium (VWR, catalog no.: 00411243) and stored at −80°C. Sections of 8 μm were cut on a CM3050 S cryostat (Leica) and stored at −80°C. Before staining, tissue sections were thawed and washed with PBS, followed by fixation with 4% PFA for 10 min. After washing with PBS, sections were incubated with permeabilization solution (Miltenyi Biotec, catalog no.: 130-126-719) for 10 min. Antibodies were diluted according to the manufacturer’s protocol in antibody staining solution (Miltenyi Biotec, catalog no.: 130-126-719). Antibodies used for IHC: EPCAM-FITC (clone: REA764, Miltenyi Biotec), CD31-APC (clone: REA730, Miltenyi Biotec), LAP-PE (clone: REA1214, Miltenyi Biotec), LAP-APC (clone: REA1214, Miltenyi Biotec), fibronectin polyclonal (RRID: AB_2547054, Thermo Fisher Scientific), Rabbit IgG (H + L)-Alexa Fluor 488 (RRID: AB_2633280), REA Control Antibody (clone: REA293, Miltenyi Biotec). Staining was performed overnight at 4°C. Images were acquired with EVOS M5000 (Thermo Fisher Scientific). Images were analyzed with ImageJ 1.53e.

Werchau N., Kotter B., Criado-Moronati E., Gosselink A., Cordes N., Lock D., Lennartz S., Kolbe C., Winter N., Teppert K., Engert F., Webster B., Mittelstaet J., Schaefer D., Mallmann P., Mallmann M.R., Ratiu D., Assenmacher M., Schaser T., von Bergwelt-Baildon M., Abramowski P, & Kaiser A.D. (2022). Combined targeting of soluble latent TGF-ß and a solid tumor-associated antigen with adapter CAR T cells. Oncoimmunology, 11(1), 2140534.

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Quantifying Aldehyde Dehydrogenase Activity

Cited 1 time

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ALDH enzymatic activity was measured using the AldeRed ALDH Detection Assay kit (Millipore) or Aldefluor assay kit (Stem Cell Technologies) following the manufacturer’s instructions. ALDH (+) cells were determined by FACS Aria II flow cytometer (BD Biosciences) or LSRII flow cytometer (BD Biosciences). Diethylamino benzaldehyde (DEAB) was added to each sample and served as a negative control for gating purpose as we have described (8 (link)). For CD133 detection, 1 million cells were harvested and incubated 50:1 with CD133/1-PE-Vio615 antibody or REA Control Antibody (Miltenyi Biotec) for 10min in the dark at 4 °C. Cells were washed with ice-cold PBS and filtered before subjected to flow cytometry analysis. Data was collected with FACSDiva software (BD Biosciences) and analyzed with FlowJo software (FlowJo LLC).

Zong X., Wang W., Ozes A., Fang F., Sandusky G.E, & Nephew K.P. (2020). EZH2-mediated Downregulation of the Tumor Suppressor DAB2IP Maintains Ovarian Cancer Stem Cells. Cancer research, 80(20), 4371-4385.

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