Histological analysis was performed as described in previous studies (48 (link), 49 (link)) with some modifications. Briefly, tissues were excised from each mouse and fixed in 4% (v/v) paraformaldehyde/PBS. After ethanol dehydration, the fixed samples were embedded in paraffin, cut into 5 μm sections with a microtome, and mounted on microscope slides (Matsunami Glass). The sections were stained with modified Mayer’s hematoxylin (Merck) and eosin Y (Wako Pure Chemical Industries Ltd). For immunohistochemistry analysis, the sections were incubated in 1% (v/v) hydrogen peroxide in methanol and treated with 10% (v/v) normal goat serum, rabbit anti-UCP1 (U6382; 1:200; Sigma–Aldrich), goat anti-rabbit IgG (Nichirei), and avidin-biotin-peroxidase complex (Nichirei).
Microscope slide
Manufactured by Matsunami
Sourced in Japan
Microscope slides are a fundamental tool used in microscopy and various scientific applications. They provide a stable and transparent platform for mounting and observing specimens under a microscope. The slides are typically made of glass or plastic and come in standardized sizes, making them compatible with most microscope models.
Automatically generated - may contain errors
Lab products found in correlation
Eosin y, by Fujifilm (1 mentions)
Modified mayer s hematoxylin, by Merck Group (1 mentions)
Avidin biotin peroxidase complex, by Merck Group (1 mentions)
Goat anti rabbit igg, by Merck Group (1 mentions)
B220 pe, by BioLegend (1 mentions)
Cd3 pe, by BioLegend (1 mentions)
Cd11b apc cy7, by BioLegend (1 mentions)
Kaluza v1, by Beckman Coulter (1 mentions)
Cytoflex, by Beckman Coulter (1 mentions)
Cytexpert software, by Beckman Coulter (1 mentions)
Coverslip, by Matsunami (1 mentions)
5 protocols using microscope slide
1
Histological Analysis of Tissue Samples
2
Fetal Liver and Spleen Analysis
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The fetal livers and spleens of transplanted embryos (E18.5) were isolated and homogenized using 18 G needles with 1 ml syringes or microscope slides (Matsunami Glass Ind., Ltd., Osaka, Japan). After ACK lysis buffer treatment, cells were stained with the following antibodies. For mouse HSC-transplanted embryos, anti-mouse CD45.1 APC or PE, CD45.2 PE/Cy7 or FITC, CD11b APC/cy7, B220 PE, and CD3 PE (BioLegend, San Diego, CA) were used. For rat HSC-transplanted embryos, anti-mouse CD45.2 FITC or PerCP and anti-rat CD45 PE (BioLegend) were used. Erythroid cells were detected from the fetal livers of rat HSC-transplanted embryos by staining with anti-mouse and anti-rat erythroid cell antibodies (BioLegend). Gallios, CytoFLEX (Beckman Coulter, Brea, CA) and Kaluza v1.3, CytExpert software (Beckman Coulter) as well as BD-LSR (BD Biosciences, San Jose, CA) and FlowJo software (TreeStar, Ashland, OR) were used for the data acquisition and analysis.
3
Histological Analysis of Adipose Tissue
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Adipose tissues and the liver were fixed in 4% paraformaldehyde and then embedded in paraffin. The tissues were cut into 5–6 μm sections using a microtome and placed on microscope slides (Matsunami Glass, Osaka, Japan). The paraffin‐embedded sections were then stained with haematoxylin and eosin. Adipocyte sizes were measured using image analysis program within the ebimage package of R/Bioconductor 14 .
4
Emulsion Particle Size Analysis via Microscopy
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The emulsion particle size was measured using optical/video microscopy
with a light microscope (objective lenses 40/0.65 and 160/0.17) interfaced
with a computer. For each test, a drop (from the storage; seeSection 5.3.4 ) was carefully
put on a microscope slide (76 mm × 26 mm, 0.3–1.0 mm;
Matsunami Glass, Osaka, Japan) and a cover slip (22 mm × 22 mm,
0.12–0.17 mm thick; Matsunami Glass, Osaka, Japan) was placed
over it immediately. Then, video and still images of the emulsion
particles were recorded. The particle sizes were determined using
ImageJ software following the image enhancement procedure recommended
by Moradi et al.15 (link) The average diameters
of the particles in the emulsions were estimated using the Sauter
mean volume diameter (d32), which is given
byeq 11 where ni is the number of droplets
counted as the ith diameter (di) of the droplet (μm). The particle
size distribution was expressed as the lognormal probability density
function f(x),eq 12 where x is the particle size, σ is the shape parameter (and is the standard deviation
of the log of the distribution), and ω is the mean of the log
of the distribution.
with a light microscope (objective lenses 40/0.65 and 160/0.17) interfaced
with a computer. For each test, a drop (from the storage; see
put on a microscope slide (76 mm × 26 mm, 0.3–1.0 mm;
Matsunami Glass, Osaka, Japan) and a cover slip (22 mm × 22 mm,
0.12–0.17 mm thick; Matsunami Glass, Osaka, Japan) was placed
over it immediately. Then, video and still images of the emulsion
particles were recorded. The particle sizes were determined using
ImageJ software following the image enhancement procedure recommended
by Moradi et al.15 (link) The average diameters
of the particles in the emulsions were estimated using the Sauter
mean volume diameter (d32), which is given
by
counted as the ith diameter (di) of the droplet (μm). The particle
size distribution was expressed as the lognormal probability density
function f(x),
of the log of the distribution), and ω is the mean of the log
of the distribution.
5
Candida Species Microscopic Characterization
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Candida species were cultured in potato dextrose agar at 36 °C for 48 h. A sterile inoculation loop was used to pick a colony of yeast up, and yeast cells were mounted in distilled water on a microscope slide (Matsunami Glass Ind., Ltd., Kishiwada, Osaka, Japan). The cells were then observed with a DIC microscopy (BX53, OLYMPUS Co., Tokyo, Japan) at magnification of 400~1000×.
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