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Anti aa4.1 pe cy7

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Anti-AA4.1 (PE-Cy7) is a fluorescent-labeled antibody for use in flow cytometry applications. It binds to the AA4.1 antigen expressed on B cell progenitors and immature B cells.

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Lab products found in correlation

Facsaria 2 instrument, by BD (1 mentions) Anti igm apc, by BD (1 mentions) Anti cd25 fitc, by BD (1 mentions) Anti b220 percp, by BD (1 mentions) Anti c kit, by BD (1 mentions) Anti cd23 fitc, by BD (1 mentions) Anti cd21 pe, by BD (1 mentions)

3 protocols using anti aa4.1 pe cy7

1

Phenotypic Analysis of Murine B Cell Subsets

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Single-cell suspensions were prepared from the femurs and spleen of 8- to 12-week-old mice as previously described (4 , 10 (link)). The scheme of Hardy (12 (link)) was used to identify immature and mature B cell subsets in the BM. The scheme by Allman et al. (9 (link)) was used to identify splenic transitional T1, T2 and splenic follicular B cells. The scheme by Loder et al. (13 (link)) was used to identify MZ B cells. A BD FACS Aria II instrument (BD Biosciences, Becton, NJ, USA) was used for sorting. Cells were washed, stained, and analyzed using the following labeled antibodies: anti-B220 (PB), anti-CD19 (APC-Cy7), anti-CD43, anti-BP-1 (PE) (APC), anti-B220 (APC), anti-IgM (APC), anti-CD23 (PE) and anti-CD21 (APC) [BD Pharmingen, San Diego, CA, USA], and anti-AA4.1 (PE-Cy7) (eBioscience, San Diego, CA, USA). Propidium iodide (PI) was used to identify dead cells. The sorting schemes are illustrated in Figure S1A in Supplementary Material.
Khass M., Blackburn T., Elgavish A., Burrows P.D, & Schroeder HW J.r. (2018). In the Absence of Central pre-B Cell Receptor Selection, Peripheral Selection Attempts to Optimize the Antibody Repertoire by Enriching for CDR-H3 Y101. Frontiers in Immunology, 9, 120.
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2

Bone Marrow B Cell Subset Analysis

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Single cell suspensions were prepared from femurs of 8 week old mice as previously described (7 ). Cells were washed, stained, and analyzed on an LSRII (BD Biosciences) using the following labeled antibodies: anti-B220 (PB), anti-CD19 (APC-Cy7), anti-CD43 (Biotin), anti-BP-1 (PE), and anti-IgM (APC) from BD Pharmingen; anti-AA4.1 (PE-Cy7) from eBioscience; and anti-IgD (Biotin) from Southern Biotech. Propidium Iodide (PI) was used to identify dead cells. The scheme of Hardy (2 (link)) was used to identify different B cell subsets in the BM. Fractions C and C’ were distinguished based on cell size.
Khass M., Blackburn T., Burrows P.D., Walter M.R., Capriotti E, & Schroeder HW J.r. (2016). VpreB serves as an invariant surrogate antigen for selecting immunoglobulin antigen-binding sites. Science immunology, 1(1), aaf6628.
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3

Isolation and Identification of B Cell Subsets

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Mononuclear cells from bone marrow and spleen were prepared as previously reported (4 (link), 18 ). B cell sub populations were separated based on expression of specific surface proteins. Early B cell subsets in the bone marrow were identified using the scheme of Melchers (19 (link)). Transitional B cell subsets were identified using the scheme of Allman (20 (link)). Marginal zone and follicular B cells were identified using the scheme of Loder (21 (link)). The following sets of monoclonal antibodies were used: Anti-B220 (PerCP) [BD Pharmingen, San Diego, CA], anti-BP-1 (PE) (a gift from John Kearney, UAB), and anti-IgM (Cy5) [Jackson ImmunoResearch], West Grove, PA], anti-cKit (allophycocyanin) [BD Pharmingen, San Diego, CA], CD19 (SPRD) [Southern Biotech, Birmingham, AL], anti IgD (biotin) [BD Pharmingen], anti CD21 (PE) [BD Pharmingen], anti-CD25 (FITC) [BD Pharmingen, San Diego, CA], anti CD21 (PE) [BD Pharmingen, San Diego, CA], anti AA4.1 (Pecy7) [ebioscience, San Diego, CA] and anti CD23 (FITC) [BD Pharmingen, San Diego, CA].
Khass M., Schelonka R.L., Liu C.R., Elgavish A., Morel L., Burrows P.D, & Schroeder HW J.r. (2017). Alterations in B cell development, CDR-H3 repertoire and dsDNA binding antibody production among C57BL/6 ΔD-iD mice congenic for the lupus susceptibility loci sle1, sle2, or sle3. Autoimmunity, 50(1), 42-51.
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