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Xpb s 19

Manufactured by Santa Cruz Biotechnology

The XPB (S-19) is a protein-specific antibody produced by Santa Cruz Biotechnology. It is designed to detect the XPB protein, which is a subunit of the general transcription factor TFIIH complex. The antibody can be used for various applications, such as Western blotting, immunoprecipitation, and immunohistochemistry, to study the expression and localization of the XPB protein.

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2 protocols using xpb s 19

1

Immunoblotting Antibody Validation

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Human Pol II (N-20) monoclonal antibody was purchased from Santa Cruz Biotechnology. 8WG16 anti-CTD (MMS-126R), H5 Ser2P-CTD (MMS-129R), and H14 Ser5P-CTD (MMS-134R) monoclonal antibodies were purchased from Covance. 3E8 Ser5P-CTD monoclonal antibodies were purchased from Millipore. TFIIB (C-18) and XPB (S-19) antibodies were purchased from Santa Cruz Biotechnology. AFF4 and CDK9 antibodies were made in-house. Anti-β-tubulin E7 monoclonal antibody was purchased from the Developmental Hybridoma Studies Bank. HCT116 cells were grown in DMEM supplemented with 10% FBS.
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2

Antibody Characterization for Epigenetic Studies

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Antibodies used in this study were DICER (3363, rabbit; Cell Signaling), ZRF1 (NBP2-12802; Novus Biologicals). RING1B clone (D22F2, 5694, rabbit; Cell Signaling), XPA (GTX103168, mouse; Genetex), XPA (FL-273, rabbit; Santa Cruz) H2B (V119 8135, mouse; Cell Signaling), FLAG (mouse; Sigma), H4K20me2 (39173, rabbit; Active Motif), H4K16ac (39930, rabbit; Active Motif), H3K36me2 (39256, rabbit; Active Motif), CPD (mouse; CosmoBio), HA (C29F4, rabbit; Cell Signaling), XPB (S-19, rabbit; Santa Cruz), MMSET (39880, mouse; Active Motif), 53BP1 (NB100-304, rabbit; Novus), and SETD8 (C18B7, rabbit; Cell Signaling).
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