Following the manufacturer’s instructions, urinary and serum 8-OHdG were analysed in duplicates using highly sensitive and competitive ELISA kits (ab201734; Abcam, Shanghai, China). Serum concentrations were determined by comparison to a standard curve and recorded in ng/l. The intra-and-inter assay coefficients of variation (CV) were 3.5% and 4.5%, respectively. Urinary 8-OHdG concentrations obtained from the standard curves were normalised to creatinine concentrations and recorded as ng/mg Cr.
Serum 8-epi-PGF2α was analysed in duplicate using competitive ELISA kits from Elabscience, Shanghai, China (cat. LogE-EL-0041). The intra-and-inter assay CV were 5.6% and 6.4%, respectively. The absorbance of both 8-epi-PGF2α and 8-OHdG was read at 450 nm on a microplate reader (Bio-Tek ELx808 microplate reader, Hayward, CA, USA).
TAC reagents were obtained from Sigma-Aldrich (Hong Kong, China). Plasma samples were thawed to measure TAC spectrophotometrically at 593 nm using Mindray BA-88A, Wuhan, Hubei, China. The estimation of TAC was based on ferric reducing ability of plasma by following the manufacturer’s instructions. The absorbance was used to obtain the concentrations after comparison to standard curves and recorded in µmol/l.
Serum 8-epi-PGF2α was analysed in duplicate using competitive ELISA kits from Elabscience, Shanghai, China (cat. LogE-EL-0041). The intra-and-inter assay CV were 5.6% and 6.4%, respectively. The absorbance of both 8-epi-PGF2α and 8-OHdG was read at 450 nm on a microplate reader (Bio-Tek ELx808 microplate reader, Hayward, CA, USA).
TAC reagents were obtained from Sigma-Aldrich (Hong Kong, China). Plasma samples were thawed to measure TAC spectrophotometrically at 593 nm using Mindray BA-88A, Wuhan, Hubei, China. The estimation of TAC was based on ferric reducing ability of plasma by following the manufacturer’s instructions. The absorbance was used to obtain the concentrations after comparison to standard curves and recorded in µmol/l.