Girard s reagent t gt

Manufactured by Merck Group

Sourced in United Kingdom

Girard's reagent T (GT) is a chemical compound used in analytical chemistry. It is a hydrazine derivative that is commonly used for the detection and quantification of carbonyl compounds, such as aldehydes and ketones, in various samples.

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2 protocols using girard s reagent t gt

Acacia Dealbata Biomass Pretreatment

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Acacia dealbata was the lignocellulosic biomass source used in this study. Branches of acacia were collected in the central region of Portugal and cut into smaller fractions, being ground and sieved to obtain a particle size of 0.25–0.84 mm.
The chemicals used in the pretreatment of acacia were [BMIM]MeSO₄ (purchased from IOLITEC), oxalic acid dihydrate (purchased from Fronteiralquimia Unipessoal, Lda), ChCl and imidazole (both purchased from Acros Organics). All the chemicals had a least 98% of purity and were used without any further purification.
For the cationization of the obtained cellulose-rich materials the following chemicals were used: lithium chloride, sodium periodate, hydroxylamine hydrochloride and Girard's reagent T (GT) (all purchased from Sigma-Aldrich). Silver nitrate and isopropanol were obtained from VWR Chemicals.

Almeida R.O., Moreira A., Moreira D., Pina M.E., Carvalho M.G., Rasteiro M.G, & Gamelas J.A. (2022). High-performance delignification of invasive tree species wood with ionic liquid and deep eutectic solvent for the production of cellulose-based polyelectrolytes. RSC Advances, 12(7), 3979-3989.

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Analytical Methods for Aldehydes Detection

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Acetone (≥ 99.8%, Honeywell, Arlington, UK), acetonitrile (ACN) (≥ 99.9%, Fisher Scientific, Loughborough, UK), methanol (MeOH) (≥ 99.9%, Honeywell, Arlington, UK, trifluoroacetic acid (TFA, 99%, Sigma-Aldrich, Dorset, UK), and formic acid (~ 98%, Fluka Analytical, Buchs, Switzerland) were HPLC grade/reagent grade. Glyoxal and methylGlyoxal (both 40% v/v in water), p-anisaldehyde (98% v/v) used as ISTD, and valeraldehyde (pentanal) (≥ 97.5% v/v) were obtained from Sigma-Aldrich, Dorset, UK. RA free serum and tissue homogenate were purchased from Golden West Biological, CA, USA. The remaining aldehyde standards, 4-hydroxyhexenal (4-HHE), 4-hydroxynonenal (4-HNE), and 4-oxo-2-nonenal (4-ONE), were obtained from Cambridge Bioscience (Cambridge, UK). Working stock solutions (1 mg mL⁻¹) of aldehyde standards were prepared in ACN and stored at − 20 °C. Girard’s reagent T (GT) (99.0–101.0% w/w), dansyl hydrazine (DH) (≥ 95% w/w), and dinitrophenyl hydrazine (DNPH) were HPLC grade obtained from Sigma-Aldrich (Dorset, UK). MALDI matrix α-cyano-4-hydroxycinnamic acid (CHCA) (≥ 98% w/w) was also obtained from Sigma-Aldrich, (Dorset, UK).

Harkin C., Smith K.W., MacKay C.L., Moore T., Brockbank S., Ruddock M, & Cobice D.F. (2022). Spatial localization of β-unsaturated aldehyde markers in murine diabetic kidney tissue by mass spectrometry imaging. Analytical and Bioanalytical Chemistry, 414(22), 6657-6670.

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