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Ms15c1

Manufactured by Thorlabs

The MS15C1 is an adjustable motorized linear stage from Thorlabs. It provides precise linear motion with a travel range of 15 mm. The stage can be controlled using a compatible Thorlabs motor controller.

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2 protocols using ms15c1

1

Nanoparticle Characterization via Photothermal Imaging

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Samples for the noncorrelative measurements were prepared by spin-coating
the particles for 20 s at 500 rpm, followed by 60 s of 4000 rpm on
an ozone-cleaned glass cover slide. After spin-coating, the samples
were sandwiched with a cavity glass slide (Thorlabs MS15C1) to provide
space for the immersion oil (n = 1.34 immersion oil
for NRs of batch 1 and n = 1.51 for the highly isotropic
spherical NPs). Nanoparticle-aggregates were excluded by postprocessing
the measurement data with a filter function for the PT signals. The
60 nm spherical particles were measured at 5.2 mW heating power at
532 nm and 0.9 mW probe power at 780 nm. The NRs (batch 1) were measured
at 2 mW heating power (660 nm) and 0.27 mW probe power (780 nm).
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2

Fluorescent Dyes and Protein Preparation

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Fluorescent dyes and proteins were prepared in phosphate-buffered saline (pH 7.4) with the final concentrations of 42.2 μM for Texas Red (sulforhodamine 101 acid chloride, 82354-19-6, Sigma-Aldrich), 50.0 μM for sulforhodamine 101 (SR 101) (60311-02-6, Sigma-Aldrich), 108 μM for Alexa Fluor 546 NHS Ester (A20002, Thermo Fisher Scientific), 44.6 μM for DsRed (rDsRed-monomer protein, 632503, Takara Bio, USA), 11.4 μM for tdTomato (tdTomato-19, Creative BioMart), and 44.8 μM for mCherry (4993, BioVision). The concentrations were calculated from the absorbance measured using an ultraviolet-visible spectrometer (Cary 300 Bio, Agilent) and the known extinction coefficients of the dyes and proteins. For quantum dot Qdot 605 (Q21701MP, Thermo Fisher Scientific), 1.0 μM solution in decane was used. For Texas Red, SR 101, Alexa Fluor 546, and Qdot 605, the sample solution filled the 600- to 800-µm-thick cavity of a glass slide (MS15C1 Thorlabs), which was then sealed by a cover glass using nail polish. For DsRed, tdTomato, and mCherry, a 120-μm sample spacer was used to hold the solution on a glass slide.
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