In situ hybridization was performed as previously described (Mahoney et al., 2014 (link)) and adapted for paraffin-embedded tissue slides. The DIG-labeled Ereg probe (Gregorieff et al., 2015 (link)) was generously supplied by Dr. Jeffrey Wrana (Lunenfeld-Tanenbaum Research Institute, Toronto, Canada). For the combined in situ/IF experiments, hybridization was carried out as above, but DIG was detected using an antibody conjugated to HRP (DIG-POD) (Roche), which was then amplified with tyramide (Perkin Elmer), and visualized with streptavidin-594 (Life Technologies). Primary incubation with the Yap antibody was carried out when the DIG-POD antibody was applied, and incubation with the secondary antibody was carried out before tyramide amplification.
Dig pod
Manufactured by Roche
The DIG-POD is a laboratory equipment product designed for sample preparation and analysis tasks. It provides a stable platform for holding and positioning various laboratory vessels and accessories during experiments or procedures. The DIG-POD's core function is to facilitate the organization and handling of samples, reagents, and other related materials in a controlled and efficient manner.
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Lab products found in correlation
2 protocols using dig pod
1
In Situ Hybridization and Immunofluorescence
2
Protein and RNA Detection Assay
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An anti‐His‐tag antibody (Nacalai Tesque) and an anti‐SUMO antibody (Life Sensors) were used for protein detection by Western blotting. An anti‐digoxigenin (DIG)‐POD (Roche) antibody was used for digoxigenin‐labeled ε RNA detection in the in vitro assay.
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