Cell-culture reagents were purchased from Gibco (Grand Island, USA). Lipofectamine and TRIzol were obtained from Invitrogen (Carlsbad, USA). Secondary antibodies for Western blotting were purchased from Li-COR, USA. The siRNA targeting Dvl2 was obtained from Gene Pharma (Shanghai, China).
Secondary antibodies for western blotting
Manufactured by LI COR
Sourced in United States
Secondary antibodies for Western blotting. These antibodies are used to detect and visualize primary antibodies that have bound to target proteins in a Western blot analysis.
Automatically generated - may contain errors
Lab products found in correlation
Cell culture reagents, by Thermo Fisher Scientific (1 mentions)
Trizol, by Thermo Fisher Scientific (1 mentions)
Lipofectamine, by Thermo Fisher Scientific (1 mentions)
Secondary antibodies for immunofluorescence, by Thermo Fisher Scientific (1 mentions)
Gamma h2ax, by Cell Signaling Technology (1 mentions)
Taqman gene probes, by Thermo Fisher Scientific (1 mentions)
2 protocols using secondary antibodies for western blotting
1
Dvl2-Targeted Gene Silencing Protocol
2
Real-time PCR and Western Blot Assays
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Real-time PCR Taqman gene probes were purchased from Life Technologies (Carlsbad, CA, USA) (MSH2: Hs00953523_m1; MLH1: Hs00179866_m1; MSH6: Hs00264721_m1; PMS2: HS00241053_m1; Rad51: Hs00153418_m1; CHEK1: Hs00967506_m1). The antibodies for PARP-1 (#9542), Caspase-3 (#9662), Rad51 (#8875), MLH1 (#3515), CHEK1 (#2360), Gamma H2A.X (#9718), MSH2 (#2850) were purchased from Cell Signaling (Danvers, MA, USA); PMS2 (#2251.00.02) and MSH6 (#2203.00.02) antibodies were purchased from Sdix (Newark, DE, USA). Antibodies targeting XPO1 (#sc-5595) and beta-actin (#sc-81178) were purchased from Santa Cruz Biotechnology. The secondary antibodies for western blotting were purchased form LI-COR (Lincoln, NE, USA) and the secondary antibodies for immunofluorescence were purchased from Life Technologies (#A11008).
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