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Orbitrap ellite mass spectrometer

Manufactured by Thermo Fisher Scientific
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The Orbitrap-Ellite mass spectrometer is a high-resolution, high-mass-accuracy mass analyzer developed by Thermo Fisher Scientific. It utilizes an elliptical Orbitrap analyzer to provide precise mass measurements of molecules. The Orbitrap-Ellite is designed for advanced applications in analytical chemistry and life sciences research.

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Lab products found in correlation

Mascot server v2, by Matrix Science (1 mentions) Proteome discoverer software v1, by Thermo Fisher Scientific (1 mentions) Orbitrap elite mass spectrometer, by Thermo Fisher Scientific (1 mentions) Rapigest, by Waters Corporation (1 mentions) Sequencing grade modified trypsin, by Promega (1 mentions)

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Orbitrap mass spectrometers (4 citations)

2 protocols using orbitrap ellite mass spectrometer

1

PRPS1 Interactome Profiling in U87 Cells

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Immunoprecipitated Flag-PRPS1 protein from glucose-deprived U87 cells was digested and analyzed by LC-MS/MS on an Orbitrap-Ellite mass spectrometer (Thermo Fisher Scientific, Waltham, MA), as described previously (16 (link)).
Qian X., Li X., Tan L., Lee J.H., Xia Y., Cai Q., Zheng Y., Wang H., Lorenzi P.L, & Lu Z. (2017). Conversion of PRPS hexamer to monomer by AMPK-mediated phosphorylation inhibits nucleotide synthesis in response to energy stress. Cancer discovery, 8(1), 94-107.
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2

Phosphoproteomics of EGF-treated PFKP

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An in vitro KAT5-acetylated purified PFKP was digested in-gel in 50 mM ammonium bicarbonate buffer containing Rapigest (Waters Corp., Milford, MA) overnight at 37°C with 200 ng of sequencing-grade modified trypsin (Promega, Madison, WI). The digest was analyzed by LC-MS/MS on an Orbitrap-Elite mass spectrometer (Thermo Fisher Scientific, Waltham, MA).
Immunoprecipitated PFKP protein from EGF-treated U251 cells was digested and analyzed by LC-MS/MS on an Orbitrap-Ellite mass spectrometer (Thermo Fisher Scientific, Waltham, MA). Proteins were identified by searching of the fragment spectra against the SwissProt protein database (EBI) using Mascot Server v.2.3 (Matrix Science, London, UK) and SEQUEST v.1.27 (University of Washington, Seattle, WA) via Proteome Discoverer software v.1.4 (Thermo Fisher Scientific). Phosphopeptide matches were analyzed by using the PhosphoRS algorithm implemented in Proteome Discoverer and manually curated (Taus et al., 2011 (link)).
Lee J.H., Liu R., Li J., Wang Y., Tan L., Li X.J., Qian X., Zhang C., Xia Y., Xu D., Guo W., Ding Z., Du L., Zheng Y., Chen Q., Lorenzi P.L., Mills G.B., Jiang T, & Lu Z. (2018). EGFR-Phosphorylated Platelet Isoform of Phosphofructokinase 1 Promotes PI3K Activation. Molecular cell, 70(2), 197-210.e7.
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