Cells were collected for protein extraction after treatment with 5 mM NaB for 24 h followed by 25 ng/ml IL-6 for 30 min. RIPA lysis buffer containing protease and phosphatase inhibitors was used to extract cellular proteins. Proteins were separated by SDS-PAGE and then transferred to PVDF membranes and probed with specific primary antibodies and secondary antibodies. An enhanced chemiluminescence detection kit (Beyotime) was used to visualize the bands. Antibodies specific for gp80 (sc-373780, 1:100) and gp130 (sc-376280, 1:100) were purchased from Santa Cruz Biotechnology (Dallas, TX, USA). Antibodies specific for STAT3 (#9139, 1:1000), JAK2 (#3230, 1:1000), p-STAT3 (#9145, 1:1000), p-JAK2 (#3771, 1:1000), and TRAF5 (#41658, 1:1000) were obtained from CST (Beverly, MA, USA). The anti-GAPDH antibody (BM1623, 1:1000), anti-β-actin antibody (BM0627, 1:1000), anti-α-tubulin antibody (BM1452, 1:1000), anti-rabbit IgG-HRP antibody (BA1054, 1:5000), and anti-mouse IgG-HRP antibody (BA1050, 1:5000) were purchased from Boster Biological Technology (Wuhan, China).
Gp130 sc 376280
Manufactured by Santa Cruz Biotechnology
Sourced in United States
Gp130 (sc-376280) is a lab equipment product offered by Santa Cruz Biotechnology. It is a recombinant protein that represents the extracellular domain of the human glycoprotein 130 (Gp130) receptor. Gp130 is a signal-transducing subunit that is shared by several cytokine receptors and plays a crucial role in signal transduction.
Automatically generated - may contain errors
Lab products found in correlation
Anti gapdh antibody bm1623, by Boster Bio (2 mentions)
Anti rabbit igg hrp antibody, by Boster Bio (2 mentions)
Anti mouse igg hrp antibody, by Boster Bio (2 mentions)
Anti β actin antibody, by Boster Bio (2 mentions)
Enhanced chemiluminescence detection kit, by Beyotime (2 mentions)
Bm0627, by Boster Bio (2 mentions)
Ba1050, by Boster Bio (2 mentions)
Ba1054, by Boster Bio (2 mentions)
Anti α tubulin antibody bm1452, by Boster Bio (1 mentions)
Anti α tubulin antibody, by Boster Bio (1 mentions)
Bm1452, by Boster Bio (1 mentions)
2 protocols using gp130 sc 376280
1
Immunoblotting of IL-6-Induced Signaling
2
IL-6 Signaling Pathway Activation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cells were collected for protein extraction after treated with 5 mM NaB for 24 hours and then 25 ng/ml IL-6 for 30 minutes. RIPA lysis buffer containing protease and phosphatase inhibitors was used to extract cellular proteins. Proteins were separated by SDS-PAGE and were then transferred to PVDF membranes and probed with speci c primary antibodies and secondary antibodies. An enhanced chemiluminescence detection kit (Beyotime) was used to visualize the bands. Antibodies speci c for gp80 (sc-373780, 1:100) and gp130 (sc-376280, 1:100) were purchased from Santa Cruz Biotechnology (Dallas, TX, USA).
Antibodies speci c for STAT3 (#9139, 1:1000), JAK2 (#3230, 1:1000), p-STAT3 (#9145, 1:1000), p-JAK2 (#3771, 1:1000) and TRAF5 (#41658, 1:1000) were obtained from CST (Beverly, MA, USA). The anti-GAPDH antibody (BM1623, 1:1000), anti-β-actin antibody (BM0627, 1:1000), anti-α-tubulin antibody (BM1452, 1:1000), anti-rabbit IgG-HRP antibody (BA1054, 1:5000) and anti-mouse IgG-HRP antibody (BA1050, 1:5000) were purchased from Boster Biological Technology (Wuhan, China).
Antibodies speci c for STAT3 (#9139, 1:1000), JAK2 (#3230, 1:1000), p-STAT3 (#9145, 1:1000), p-JAK2 (#3771, 1:1000) and TRAF5 (#41658, 1:1000) were obtained from CST (Beverly, MA, USA). The anti-GAPDH antibody (BM1623, 1:1000), anti-β-actin antibody (BM0627, 1:1000), anti-α-tubulin antibody (BM1452, 1:1000), anti-rabbit IgG-HRP antibody (BA1054, 1:5000) and anti-mouse IgG-HRP antibody (BA1050, 1:5000) were purchased from Boster Biological Technology (Wuhan, China).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!