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Nigericin n7143

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Nigericin (N7143) is a chemical compound produced by Streptomyces hygroscopicus. It functions as a potassium ionophore, facilitating the exchange of potassium ions across cell membranes.

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Lab products found in correlation

M csf 216 mc, by R&D Systems (1 mentions) Alum 77161, by Thermo Fisher Scientific (1 mentions) Anti gsdmd ab209845, by Abcam (1 mentions) Anti caspase 1 ag 20b 0042, by Adipogen (1 mentions) Poly da dt tlrl patn, by InvivoGen (1 mentions) Lipofectamine rnaimax transfection reagent, by Thermo Fisher Scientific (1 mentions) Anti brd4 a301 985a, by Fortis Life Sciences (1 mentions) Il 6 88 7064 22 elisa kits, by Thermo Fisher Scientific (1 mentions) Anti asc ag 25b 0006 c100, by Adipogen (1 mentions) Lps escherichia coli o111 b4 l2630, by Merck Group (1 mentions) Anti h3k27ac ab4729, by Abcam (1 mentions) Anti β actin hrp, by Merck Group (1 mentions) Af 201 na, by R&D Systems (1 mentions) Lps escherichia coli 026 b6, by Merck Group (1 mentions) Phosphatase inhibitor cocktail 78420, by Thermo Fisher Scientific (1 mentions) Anti human il 1β, by R&D Systems (1 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)

4 protocols using nigericin n7143

1

Inflammatory Stimuli Reagents Protocol

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LPS (L3024), ATP (A7699) and nigericin (N7143) were purchased from Sigma-Aldrich (St. Louis, MO, USA). MSU (tlr-msu) was purchased from Invivogen (San Diego, CA, USA). Alum (77161) was purchased from Thermo-Scientific (Thermo Fisher Scientific, MA, USA). M-CSF (216-MC) was purchased from R&D Systems (Lille, France). Recombinant HSP70 was kindly provided by Carmen Garrido’s team.
Martine P., Chevriaux A., Derangère V., Apetoh L., Garrido C., Ghiringhelli F, & Rébé C. (2019). HSP70 is a negative regulator of NLRP3 inflammasome activation. Cell Death & Disease, 10(4), 256.
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2

LPS, Nigericin, and DOTAP Modulation of Inflammatory Responses

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LPS (Escherichia coli O111:B4, L2630), nigericin (N7143) and N-(2,3-dioleoyloxy-1-propyl)trimethylammonium methyl sulfate (DOTAP) liposomal transfection reagent (144189-73-1) were from Sigma-Aldrich. Ultrapure flagellin from S. typhimurium (tlrl-epstfla-5) and poly(dA:dT; tlrl-patn) were purchased from InvivoGen. TcdB from List Labs (155). Lipofectamine RNAiMAX Transfection Reagent (13778150) was from Invitrogen. Mouse TNF-α (88-7324-88), IL-1β (88-7013-88), IL-18 (BMS618-3), and IL-6 (88-7064-22) ELISA kits were from Invitrogen. The LightShift Chemiluminescent EMSA Kit (20148) was from Thermo Fisher Scientific Inc. Anti–IL-1β (AF-401-NA) was from R&D Systems. Anti–caspase-1 (AG-20B-0042) and anti-ASC (AG-25B-0006-C100) were from AdipoGen. Anti-GSDMD (ab209845) and anti-H3K27ac (ab4729) were from Abcam. Antiactin (sc-47778), anti-IRF8 (sc-365042), anti-RNAPII (sc-47701), and anti-PU.1 (sc-390405) were from Santa Cruz Biotechnology, Inc. Anti-Brd4 (A301-985A) was from Bethyl Laboratories. Anti-H3K4me1 (07-436) and anti-H3K4me3 (05-745R) were from EMD Millipore.
Dong X., Hu X., Bao Y., Li G., Yang X.D., Slauch J.M, & Chen L.F. (2021). Brd4 regulates NLRC4 inflammasome activation by facilitating IRF8-mediated transcription of Naips. The Journal of Cell Biology, 220(3), e202005148.
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3

Investigating Cytokine Responses in LPS-Stimulated Cells

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LPS (Escherichia coli 026:B6), adenosine 5′-triphosphate disodium salt hydrate (A2383; ATP), nigericin (N7143), and penicillin–streptomycin (Pen/Strep, P4333) were purchased from Sigma-Aldrich. Human IFN Beta (Beta 1a, Mammalian Expressed) (11415-1) and Human IFN Alpha Hybrid (Universal Type I IFN) (11200-1) were obtained from PBL Assay Science. Phosphatase inhibitor cocktail (78420) was sourced from Thermo Fisher Scientific, and FBS was obtained from Gibco.
Primary antibodies for Western blot analysis and their final concentrations were as follows: anti-human IL-1β (0.1 μg/ml, goat polyclonal, AF-201-NA; R&D Systems) and anti-β-actin HRP (0.2 μg/ml, mouse monoclonal, A3854; Sigma-Aldrich).
Díaz-Pino R., Rice G.I., San Felipe D., Pepanashvili T., Kasher P.R., Briggs T.A, & López-Castejón G. (2024). Type I interferon regulates interleukin-1beta and IL-18 production and secretion in human macrophages. Life Science Alliance, 7(6), e202302399.
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4

Immune Stimulant Compound Assay

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LPS (L3024), ATP (A7699) and nigericin (N7143) were purchased from Sigma-Aldrich. SiO2 (tlrl-sio), MSU (tlrl-msu) and CPPD (tlrl-cppd) were purchased from InvivoGen.
Chevriaux A., Pilot T., Derangère V., Simonin H., Martine P., Chalmin F., Ghiringhelli F, & Rébé C. (2020). Cathepsin B Is Required for NLRP3 Inflammasome Activation in Macrophages, Through NLRP3 Interaction. Frontiers in Cell and Developmental Biology, 8, 167.
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