GM12878 cells were obtained from Coriell Institute for Medical Research. Cell line was grown in RPMI 1640 media (11875–093, Gibco) supplemented by 15% Fetal Bovine Serum (26140–079, Gibco) and 1% penicillin-streptomycin (15140–122, Gibco) at 37°C and 5% CO2, passaged every 2–3 days to maintain exponential growth. Before the scRNA-seq experiment, the concentration of the cell suspension was adjusted to 3.2 × 105 /mL in PBS using a hemocytometer to facilitate single cell trapping.
MEF cells were obtained from ATCC (SCRC-1040) and cultured in DMEM (ATCC 30–2002) with 15% FBS and 1%PS at 37°C and 5% CO2. Cells were harvested at 80% confluence. They were detached by incubating with 0.25% trypsin with 0.1% EDTA (Thermo Fisher 25200056) for 1 min and then centrifuged at 120 × g for 5 min. Then, the supernatant was discarded, and cells were resuspended in fresh media and then adjusted to a concentration of 3.2 × 105 /ml.
MEF cells were obtained from ATCC (SCRC-1040) and cultured in DMEM (ATCC 30–2002) with 15% FBS and 1%PS at 37°C and 5% CO2. Cells were harvested at 80% confluence. They were detached by incubating with 0.25% trypsin with 0.1% EDTA (Thermo Fisher 25200056) for 1 min and then centrifuged at 120 × g for 5 min. Then, the supernatant was discarded, and cells were resuspended in fresh media and then adjusted to a concentration of 3.2 × 105 /ml.