Anti mouse cd68 apc

Paraffin-embedded muscle tissues were sectioned at 10-μm thick, stained with hematoxylin and eosin (H&E), and imaged on a Nikon Eclipse 80i, Nikon Plan Apochromat 20x objective lens (numerical aperture 0.75). For immunofluorescence microscopy, muscle tissue was sectioned at 10-μm-thickness by cryostat. Sections were incubated with CD31-FITC (1:100; Thermo Fisher, 11-0311-85), anti-mouse CD68-APC (1:100; BioLegend, 137008), or anti-mouse α-Smooth Muscle Actin-Cy3 (1:100; MilliporeSigma, C6198), and then mounted with 4′,6-Diamidino-2-phenylindole dihydrochloride (DAPI) (Thermo Fisher, P36931). Using a Nikon Eclipse 80i inverted microscope with Nikon Plan Apochromat 10x objective lens (numerical aperture 0.3), 6 fluorescence images were acquired in the regions of interest as determined by cellular infiltration using the DAPI staining. Quantification of cell types was expressed as a ratio of each DAPI⁺ cellular marker (CD68, CD31, SMA) to the total DAPI stained cells in the field. Each individual data point represented one animal and reflected the average of 3–4 images captured from adjacent tissue sections per slide. All subsequent analyses were performed by Image J software(Schneider et al., 2012 (link)).