Mocetinostat

Manufactured by Cayman Chemical

Sourced in United States

Mocetinostat is a chemical compound used as a tool compound for in vitro research. It is a histone deacetylase (HDAC) inhibitor that can be used to study the effects of HDAC inhibition in various experimental systems.

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Lab products found in correlation

Fetal bovine serum (fbs), by Atlanta Biologicals (2 mentions) Puromycin, by Lonza (1 mentions) Entinostat, by Cayman Chemical (1 mentions) Sodium butyrate, by Merck Group (1 mentions) Rpmi 1640 medium, by Lonza (1 mentions) Penicillin, by Lonza (1 mentions) Streptomycin, by Lonza (1 mentions) Gentamicin, by Lonza (1 mentions) Ms 275, by Santa Cruz Biotechnology (1 mentions) Pronase, by Merck Group (1 mentions) Trichostatin a, by Cayman Chemical (1 mentions) Tmp195, by Cayman Chemical (1 mentions) Rgfp966, by Cayman Chemical (1 mentions) Brd6688, by Cayman Chemical (1 mentions) Cycloheximide (chx), by Merck Group (1 mentions) Vorinostat, by Cayman Chemical (1 mentions) Pomalidomide, by Cayman Chemical (1 mentions) Lipopolysaccharides (lps), by Merck Group (1 mentions) Dexamethasone (dex), by Merck Group (1 mentions)

4 protocols using mocetinostat

Cell Culture Reagents and Compound Preparation

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Cell-culture reagents including RPMI 1640 medium (without phenol red for HTS) and antibiotics (penicillin, streptomycin, puromycin, and gentamicin) were purchased from Lonza (Allendale, NJ, USA), Santa Cruz Biotechnology (Dallas, TX, USA), and Fisher Scientific (Pittsburgh, PA, USA). Fetal bovine serum (FBS) was obtained from Atlanta Biologicals (Flowery Branch, GA, USA). Sodium butyrate was procured from MilliporeSigma (St. Louis, MO, USA), while mocetinostat, entinostat, and tucidinostat were obtained from Cayman Chemical (Ann Arbor, MI, USA). Sodium butyrate was dissolved in RPMI 1640, while all other chemicals were dissolved in dimethyl sulfoxide (DMSO). An equal volume of RPMI 1640 or DMSO was used in all cell-culture experiments as a negative control.

Lyu W., Mi D., Vinson P.N., Xiao Y, & Zhang G. (2022). Large-Scale Identification of Multiple Classes of Host Defense Peptide-Inducing Compounds for Antimicrobial Therapy. International Journal of Molecular Sciences, 23(15), 8400.

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Modulation of HDAC and NLRP3 Pathways

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Pan-HDAC inhibitor Trichostatin A (TSA), class IIa HDAC inhibitors (HDAC4,5,7 and 9) TMP195, Class I & IV HDAC inhibitor (HDAC1,2,3 and 11) Mocetinostat, HDAC6 and 8 inhibitor BRD73954, HDAC3 inhibitor RGFP966 and a HDAC1 and 2 inhibitor BRD6688 were purchased from Cayman Chemical, HDAC1 and 3 inhibitor MS-275, and HDAC1 inhibitor 4-(dimethylamino)-N-[6-(hydroxyamino)-6-oxohexyl]-benzamide (DHOB) were purchased from Santa Cruz Biotechnology, NLRP3 specific inhibitor CP-456773/MCC 950, caspase-1 inhibitor caspase-1 Inhibitor II and protein synthesis inhibitor, cycloheximide, and pronase were purchased from Sigma-Aldrich. The stocks of all the chemical inhibitors were prepared in DMSO following the instructions of the vendors and stored at -80°C before use.

Moreira J.D., Iakhiaev A., Vankayalapati R., Jung B.G, & Samten B. (2022). Histone deacetylase-2 controls IL-1β production through the regulation of NLRP3 expression and activation in tuberculosis infection. iScience, 25(8), 104799.

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Cell Culture Reagents and Epigenetic Modulators

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RPMI 1640 medium, penicillin, streptomycin, and puromycin were purchased from Hyclone (Logan, UT), while fetal bovine serum (FBS) was obtained from Atlanta Biologicals (Lawrenceville, GA). Sodium butyrate and bacterial lipopolysaccharide (LPS) from Escherichia coli O55:B5 was procured from MilliporeSigma (St. Louis, MO), and BIX01294, A-366, UNC1999, SGI-1027, 5-azacytidine (AZA), vorinostat, and mocetinostat (Figure 1) were acquired from Cayman Chemical (Ann Arbor, Michigan). Sodium butyrate and LPS were dissolved in RPMI 1640, while all other chemicals were dissolved in dimethyl sulfoxide (DMSO). In all subsequent cell culture experiments, cells treated with an equal volume of RPMI 1640 or DMSO were used as negative controls to the cells treated with individual compounds or their combinations.

Whitmore M.A., Li H., Lyu W., Khanam S, & Zhang G. (2022). Epigenetic Regulation of Host Defense Peptide Synthesis: Synergy Between Histone Deacetylase Inhibitors and DNA/Histone Methyltransferase Inhibitors. Frontiers in Immunology, 13, 874706.

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Zebrafish Larval Drug Treatments

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Detailed methods are provided in the online version of this paper and include the following: Drug treatment of whole larvae Dexamethasone (Sigma, Gillingham, UK) was dissolved in DMSO to a stock concentration of 5 mM. The working concentration was 10 mM prepared by dilution from stock solution in fish water. Lipopolysaccharides from Escherichia coli O55:B5 (LPS, Sigma) were dissolved in PBS to a stock concentration of 1 mg/ml. The working dilution was 50 mg/ml. Pomalidomide (Cayman Chemicals, Michigan, USA) was diluted in DMSO at a stock concentration of 10 mg/ml. For the treatments, 6.9 ml of the stock where diluted in 1.5 ml of fish water. Mocetinostat (Cayman Chemicals, Ann Arbor, MI, USA) was used at a concentration of 1 mM; TSA (Sigma) was used at 200 nM. Larvae were pre-treated for 2 h before the injury and were incubated for 24 and 48 hpl. Larvae were collected from the breeding tanks and were randomly divided into Petri dishes at a density of maximally 30 larvae per dish, but no formal randomization method was used. For most drug treatments, larvae were incubated with the drug from 3 dpf until 5 dpf, if not indicated differently.

Cavone L., McCann T., Drake L.K., Aguzzi E.A., Oprişoreanu A.M., Pedersen E., Sandi S., Selvarajah J., Tsarouchas T.M., Wehner D., Keatinge M., Mysiak K.S., Henderson B.E.P., Dobie R., Henderson N.C., Becker T, & Becker C.G. (2021). A unique macrophage subpopulation signals directly to progenitor cells to promote regenerative neurogenesis in the zebrafish spinal cord. Developmental cell, 56(11).

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