AD5 and cyclin D1 were purified as previously described (13 (link),21 ). A spectroscopic sample of AD5 was prepared in phosphate-buffered solution (PBS) at pH 7.4. The concentration of purified AD5 and cyclin D1 was determined using a Bradford assay (Sangon Biotech Co., Ltd., Shanghai, China), according to the manufacturer's protocol. The anti-V5-tag antibody (cat. no. M1008-2) was purchased from Hangzhou HuaAn Biotechnology Co., Ltd. (Hangzhou, China). Horseradish peroxidase (HRP)-conjugated goat anti-mouse immunoglobulin G (IgG) (cat. no. SA00001-1) was purchased from the ProteinTech Group, Inc. (Chicago, IL, USA). Bovine serum albumin (BSA) and o-phenylenediamine (OPD) were purchased from Sigma-Aldrich; Merck KGaA (Darmstadt, Germany). CuCl2 and FeCl3 were purchased from Beijing Chemical Works (Beijing, China). All other chemicals were of analytical grade.
Fecl3
Manufactured by Beijing Chemical Works
Sourced in China
FeCl3, or ferric chloride, is a chemical compound that is commonly used as a laboratory reagent. It is a crystalline solid that is soluble in water and has a distinctive yellow-brown color. FeCl3 is a powerful oxidizing agent and is often used in analytical chemistry, water treatment, and the production of other chemical compounds.
Automatically generated - may contain errors
Lab products found in correlation
Sodium hydroxide (naoh), by Beijing Chemical Works (2 mentions)
Hydrochloric acid (hcl), by Beijing Chemical Works (2 mentions)
Bradford assay, by Sangon (1 mentions)
Cucl2, by Beijing Chemical Works (1 mentions)
Bovine serum albumin (bsa), by Merck Group (1 mentions)
Feso4, by Beijing Chemical Works (1 mentions)
3 protocols using fecl3
1
Purification and Characterization of AD5 and Cyclin D1
2
Luffa Leaf Adsorption Efficiency
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In 2022, Luffa leaf (LL) was obtained from the experimental area of Jilin Agricultural University (Changchun, China), washed, dried, and crushed. NaOH, HCl, KOH, FeSO4, and FeCl3 were purchased from Beijing Chemical Works (Beijing, China) and used without further purification. RhB (CAS: 81-88-9, ≥95%) and TH (CAS: 64-75-5, 96%) were supplied by Aladdin Chemical (Shanghai) Co., Ltd. (Shanghai, China), and the structural formulas are shown in Figure S1 (Supplementary Material ).
3
Enzymatic Hydrolysis of Cellulose
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T. reesei was extracted and preserved from decayed straw by the Key Laboratory of Straw Comprehensive Utilization and Black Soil Conservation, Ministry of Education, China.
The potato was bought from the local vegetable market. D-glucose monohydrate, FeCl3, HCl, H2O2, and NaOH were supplied by Beijing Chemical Works (Beijing, China). Malachite green (MG), tetracycline hydrochloride (TH), t-Butanol, and p-Benzoquinone were supplied by Aladdin Chemical (Shanghai) Co., Ltd. (Shanghai, China). All the above reagents were of analytical purity grade and did not require further purification. The water involved in the rinsing and preparation process was deionized water.
The potato was bought from the local vegetable market. D-glucose monohydrate, FeCl3, HCl, H2O2, and NaOH were supplied by Beijing Chemical Works (Beijing, China). Malachite green (MG), tetracycline hydrochloride (TH), t-Butanol, and p-Benzoquinone were supplied by Aladdin Chemical (Shanghai) Co., Ltd. (Shanghai, China). All the above reagents were of analytical purity grade and did not require further purification. The water involved in the rinsing and preparation process was deionized water.
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