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Cfw 1308c color digital camera

Manufactured by Techcomp Instruments
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The CFW-1308C is a color digital camera designed for laboratory applications. It features a CMOS image sensor with a resolution of 1.3 megapixels and the ability to capture images in 24-bit color depth. The camera is capable of providing a maximum frame rate of 30 frames per second at its full resolution.

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Lab products found in correlation

Dm4000b microscope, by Leica (2 mentions) Tumor necrosis factor (tnf), by R&D Systems (1 mentions) May gr nwald and giemsa solutions, by Merck Group (1 mentions) Cd163 clone ghi 61, by BioLegend (1 mentions) Cd1a clone hi149, by BioLegend (1 mentions) Cd16 clone 3g8, by BioLegend (1 mentions) Interleukin 4 (il 4), by Miltenyi Biotec (1 mentions) Alexa fluor 488 or 647 conjugated goat anti mouse igg, by Thermo Fisher Scientific (1 mentions) Lsm 710, by Zeiss (1 mentions)

Close spelling variants of this product

CFW-1308C (5 citations) Digital camera (3 citations)

2 protocols using cfw 1308c color digital camera

1

Monocyte-Derived Macrophage and Dendritic Cell Culture

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Monocytes were isolated from cryopreserved PBMCs as described above and cultured for 5 d in complete RPMI with 10% FBS at 106 cells/ml in the presence of 100 ng/ml M-CSF, 40 ng/ml of IL-4 (Miltenyi Biotec), and 5 ng/ml TNF (R&D Systems). After culture, cells exhibiting the macrophage or DC phenotype were quantified using flow cytometry staining for CD16 (clone 3G8; BioLegend) or CD1a (clone HI149; BioLegend) markers, respectively. CD163 (clone GHI/61; BioLegend) and viability dye (DAPI) were also included in the staining panel, and the staining buffer was composed of PBS with 0.5% human serum and 2 mM EDTA. For morphological analysis, cells were cytospun, stained with May–Grünwald and Giemsa solutions (Sigma-Aldrich), and imaged using a CFW-1308C color digital camera (Scion Corporation) on a Leica DM 4000 B microscope (Leica Microsystems).
Cepika A.M., Banchereau R., Segura E., Ohouo M., Cantarel B., Goller K., Cantrell V., Ruchaud E., Gatewood E., Nguyen P., Gu J., Anguiano E., Zurawski S., Baisch J.M., Punaro M., Baldwin N., Obermoser G., Palucka K., Banchereau J., Amigorena S, & Pascual V. (2017). A multidimensional blood stimulation assay reveals immune alterations underlying systemic juvenile idiopathic arthritis. The Journal of Experimental Medicine, 214(11), 3449-3466.
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2

Immunophenotyping of Immune Cells in Tissue

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Paraffin-embedded and formalin-fixed samples were cut into 5 μM sections, which were then processed for IF staining or H&E staining. Immunofluorescence was performed with antibodies to pan-TCRγδ, IL-17A, IL-23p19, CD11c, CD68, ZO-1, Claudin-2, and pan-Cytokeratin. Secondary antibodies were Alexa Fluor 488- or 647-conjugated goat anti–mouse IgG or Dylight 488- or 649-conjugated goat anti-rabbit IgG (Invitrogen). Images were acquired with a confocal microscopy (Zeiss LSM 710, Carl Zeiss). For morphological analysis, sorted inf-DCs, inf-macrophages, or PMN-MDSCs were subjected to cytospin and stained with May-Grunwald/Giemsa. Pictures were taken with a CFW-1308C color digital camera (Scion Corporation) on a Leica DM 4000 B microscope.
Wu P., Wu D., Ni C., Ye J., Chen W., Hu G., Wang Z., Wang C., Zhang Z., Xia W., Chen Z., Wang K., Zhang T., Xu J., Han Y., Zhang T., Wu X., Wang J., Gong W., Zheng S., Qiu F., Yan J, & Huang J. (2014). γδT17 Cells Promote the Accumulation and Expansion of Myeloid-Derived Suppressor Cells in Human Colorectal Cancer. Immunity, 40(5), 785-800.
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