Celltitre glo 3d cell viability assay

Manufactured by Promega

CellTiter-Glo 3D Cell Viability Assay is a luminescent cell viability assay that quantifies ATP, which is an indicator of metabolically active cells. The assay is designed for use with 3D cell culture models.

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Lab products found in correlation

Celltiter glo luminescent assay, by Promega (2 mentions) Pen strep, by Thermo Fisher Scientific (1 mentions) Rpmi 1640 media, by Thermo Fisher Scientific (1 mentions) Hites media, by American Type Culture Collection (1 mentions)

Close spelling variants of this product

CellTitre-Glo® Luminescent 3D Cell Viability Assay CellTitre-Glo cell viability assay CellTitre-Glo 3D 3D Cell Viability Assay CellTitre-Glo assay CellTitre-Glo

3 protocols using celltitre glo 3d cell viability assay

Cellular Viability Assays: 2D and 3D

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2D cell viability was assessed using CellTiter-Glo Luminescent Assay (Promega, Madison, WI) according to manufacturer’s instructions. 3D cell viability was determined with CellTitre-Glo 3D Cell Viability Assay (Promega, Madison, WI), according to manufacturer’s instructions.

Crawford N., Stott K.J., Sessler T., McCann C., McDaid W., Lees A., Latimer C., Fox J.P., Munck J.M., Smyth T., Shah A., Martins V., Lawler M., Dunne P.D., Kerr E.M., McDade S.S., Coyle V.M, & Longley D.B. (2021). Clinical Positioning of the IAP Antagonist Tolinapant (ASTX660) in Colorectal Cancer. Molecular Cancer Therapeutics, 20(9), 1627-1639.

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Cell Viability Assays in 2D and 3D

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Two-dimensional cell viability was assessed using CellTiter-Glo Luminescent Assay (Promega) according to the manufacturer's instructions. Three-dimensional (3D) cell viability was determined with CellTitre-Glo 3D Cell Viability Assay (Promega), according to the manufacturer's instructions.

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Comparative Cell Viability Assay

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LuCaP PDX cells (2.0X10⁴) and NCI-H660 cells (5.0x10³) were seeded in 96-well plates in either phenol red-free RPMI 1640 media (Gibco) supplemented with 5% charcoal/dextran treated fetal bovine serum (Atlanta Biologicals) and 1X pen/strep (Gibco) or HITES media (ATCC). Cells were treated 4–6 hours after seeding with vehicle, AMG-337 (50 nM) or cabozantinib (2.5 μM) in three replicate wells. Viability was assessed at time 0 and 72 hours post treatment using the CellTitre Glo 3D-Cell Viability Assay (Promega) according to manufacturer’s protocols.

Labrecque M.P., Brown L.G., Coleman I.M., Nguyen H.M., Lin D.W., Corey E., Nelson P.S, & Morrissey C. (2021). Cabozantinib can block growth of neuroendocrine prostate cancer patient-derived xenografts by disrupting tumor vasculature. PLoS ONE, 16(1), e0245602.

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