Total RNA samples were prepared using the TRI Reagent (SIGMA), according to the manufacturer’s recommended procedure. Reverse transcription and PCR amplification were carried out as described previously [54] (link). The gene specific primers used in current study are summarized in Table S1. PCR products were subjected to 2% agarose gel electrophoresis and the gels were viewed by means of an Image Analyzer (model LAS-4000mini, GE Healthcare, Tokyo, Japan) after staining with ethidium bromide.
Image analyzer
Manufactured by GE Healthcare
Sourced in Japan, United States
The Image Analyzer is a lab equipment product that provides digital image analysis capabilities. It is designed to capture, process, and analyze images obtained from various imaging modalities. The core function of the Image Analyzer is to enable users to quantify and extract relevant data from digital images, supporting various applications in the healthcare and research domains.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using image analyzer
1
RNA Extraction and RT-PCR Analysis
2
Western Blot Protein Analysis
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Plant materials were ground, and total proteins were extracted in a buffer (50 mM Tris-HCl, 100 mM DTT, pH 7.5, 1 mM EDTA, 250 mM sucrose, 5 mM leupeptin and 100 mM PMSF). A SDS-12% polyacrylamide gel electrophoresis was used to separate proteins, after which proteins on the gel were blotted to a polyvinylidene difluoride membrane. The primary antibody specific to each protein was incubated with the membrane. After binding the secondary antibody with horse radish peroxidase, chemiluminescence signals were detected with an image analyzer (GE Healthcare Life Sciences, USA). Ponceau-S solution containing 5% acetic acid and 0.1% Ponceau-S was used to stain the total proteins on the membrane.
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