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Total irak4

Manufactured by Cell Signaling Technology
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Total IRAK4 is a quantitative sandwich ELISA kit that measures the total amount of IRAK4 protein in cell and tissue lysates. IRAK4 is a serine/threonine protein kinase that plays a critical role in the interleukin-1 receptor (IL-1R) and Toll-like receptor (TLR) signaling pathways.

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Lab products found in correlation

Total p38α mapk, by Cell Signaling Technology (2 mentions) Gapdh, by Cell Signaling Technology (2 mentions) P38α mapk, by Cell Signaling Technology (1 mentions)

2 protocols using total irak4

1

Antibodies for Signaling Pathway Analysis

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Antibodies recognizing p105/NFκB1 phosphorylated at Ser933, p38α MAPK phosphorylated at its Thr-Gly-Tyr motif, total p38α MAPK, total IRAK4 and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) were from Cell Signalling Technology. An antibody recognizing IRAK4 phosphorylated at both Thr345 and Ser346 was provided by Vikram Rao, Pfizer Inc. (31 (link)). An antibody recognizing JNKs phosphorylated at their Thr-Pro-Tyr motif was from Invitrogen, and a rabbit secondary antibody conjugated to horseradish peroxidase (HRP) was from Pierce.
Nanda S.K., Lopez-Pelaez M., Arthur J.S., Marchesi F, & Cohen P. (2016). Suppression of IRAK1 or IRAK4 Catalytic Activity, but Not Type 1 IFN Signaling, Prevents Lupus Nephritis in Mice Expressing a Ubiquitin Binding–Defective Mutant of ABIN1. The Journal of Immunology Author Choice, 197(11), 4266-4273.
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2

Antibody Validation for Signaling Proteins

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Abs recognizing p105/NFκB1 phosphorylated at Ser933, p38α MAPK phosphorylated at its Thr-Gly-Tyr motif, total p38α MAPK, total IRAK4, and GAPDH were from Cell Signaling Technology. An Ab recognizing IRAK4 phosphorylated at Thr345 and Ser346 was provided by V. Rao (Pfizer) (31 (link)). An Ab recognizing JNKs phosphorylated at their Thr-Pro-Tyr motif was from Invitrogen, and a rabbit secondary Ab conjugated to HRP was from Pierce.
Nanda S.K., Lopez-Pelaez M., Arthur J.S., Marchesi F, & Cohen P. (2016). Suppression of IRAK1 or IRAK4 Catalytic Activity, but Not Type 1 IFN Signaling, Prevents Lupus Nephritis in Mice Expressing a Ubiquitin Binding–Defective Mutant of ABIN1. The Journal of Immunology Author Choice, 197(11), 4266-4273.
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