V plex chemokine panel 1 human kit

The concentrations of cytokines and chemokines were assessed by V-PLEX Proinflammatory Panel 1 Human Kit, V-PLEX Chemokine Panel 1 Human Kit (K15049D), U-PLEX Interferon Combo kit (K15094K), and V-PLEX Chemokine Panel 1 Human Kit (K15047D) from Meso Scale Discovery. The kits utilize multiplexed electrochemiluminescence, and light intensity was measured by SECTOR Imager S 600. The assays were performed according to the manufacturer’s protocol.

Plasma samples from baseline (pre-ART) were thawed from storage at −80 °C. Thawed samples were then filtered, aliquoted, and frozen again for storage at −80 °C until ready for use to minimise subsequent freeze–thaw cycles during analysis. Using Meso Scale Discovery (MSD) multiplexed immunoassay kits as per the manufacturer's recommendations (www.mesoscale.com) we quantified: V-PLEX Proinflammatory Panel 1 Human Kit (K15049D; interferon γ [IFN-γ], interleukin [IL]-1β, IL-2, IL-6, IL-8, IL-10, IL-13, tumour necrosis factor α [TNF-α]), V-PLEX Cytokine Panel 1 Human Kit (K15050D; GM-CSF, IL-1α, IL-12/IL-23p40, IL-15, IL-16, IL-17A, TNF-β, VEGF-A), and V-PLEX Chemokine Panel 1 Human Kit (K15047D; eotaxin (CCL-11), macrophage inflammatory protein [MIP]-1β/CCL4, TARC (CCL-17), CXCL-10, MIP-1α/CCL3, IL-8, monocyte chemoattractant protein [MCP]-1/CCL2, myeloid dendritic cell [MDC/CCL22], MCP-4/CCL3). For soluble CD14 (sCD14) and IL-1 R1 analysis, R&D Systems Human CD14 DuoSet enzyme-linked immunosorbent assay (ELISA) (DY383) and Human IL-1 RI DuoSet ELISA (DY269) were used, respectively, as per manufacturer's recommendations (www.rndsystems.com). SoftMax Pro 5.3 was used to acquire and compute concentration values. Laboratory procedures were described in detail by Manabe et al.²¹ (link)