Cell viability was assessed with a Cell Counting Kit-8 assay (Dojindo, Kumamoto, Japan). Briefly, H9c2 cells were seeded in 96-well plates (NEST Biotechnology) at a density of 5000 cells/well in DMEM with 10% FBS. After being performed the indicated treatments, cells were incubated with 110 μl of CCK-8 solution (serum-free DMEM/CCK-8 reagent = 10/1) per well for 2 h. Finally, the absorbance value of each well was measured at a wavelength of 450 nm with a Microplate ReaderBio-Rad microplate reader (Model 680; Bio Rad Laboratories, Inc., Hercules, CA, USA).
Microplate readerbio rad microplate reader model 680
Manufactured by Bio-Rad
Sourced in United States
The Bio-Rad Microplate Reader (Model 680) is a versatile instrument designed for the measurement of absorbance in microplates. It can perform a wide range of photometric measurements, including endpoint, kinetic, and spectral scanning analyses.
Automatically generated - may contain errors
Lab products found in correlation
96 well plate, by NEST Biotechnology (2 mentions)
Cell counting kit 8 (cck8), by Dojindo Laboratories (1 mentions)
Annexin 5 fitc apoptosis detection kit, by Dojindo Laboratories (1 mentions)
Lsrfortessa, by BD (1 mentions)
Cell counting kit 8 cck 8, by Dojindo Laboratories (1 mentions)
Cell counting kit 8 assay, by Dojindo Laboratories (1 mentions)
3 protocols using microplate readerbio rad microplate reader model 680
1
Cell Viability Assay with CCK-8
2
Proliferation and Apoptosis of HUVECs
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The proliferation and viability of HUVECs was measured by a Microplate ReaderBio-Rad microplate reader (Model 680; Bio Rad Laboratories, Inc., Hercules, CA, USA) at 450 nm using the Cell Counting Kit-8 (CCK-8, Dojindo, Kumamoto, Japan), following the manufacturer's instructions.
Cell apoptosis was analyzed by flow cytometry using the Annexin V-FITC Apoptosis Detection Kit (dojindo laboratories, Kumamoto, Japan) according to the manufacturer’s instruction. Flow cytometry was performed using the LSRFortessa (BD) and flow data was analyzed using FlowJo v.10 (Tree Star).
Cell apoptosis was analyzed by flow cytometry using the Annexin V-FITC Apoptosis Detection Kit (dojindo laboratories, Kumamoto, Japan) according to the manufacturer’s instruction. Flow cytometry was performed using the LSRFortessa (BD) and flow data was analyzed using FlowJo v.10 (Tree Star).
3
Cell Viability Quantification with CCK-8
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cell viability was assessed with a Cell Counting Kit-8 assay (Dojindo, Kumamoto, Japan). Brie y, H9c2 cells were seeded in 96-well plates (NEST Biotechnology) at a density of 5000 cells/well in DMEM with 10% FBS. After being performed the indicated treatments, cells were incubated with 110 μl of CCK-8 solution (serum-free DMEM/CCK-8 reagent = 10/1) per well for 2 hours. Finally, the absorbance value of each well was measured at a wavelength of 450 nm with a Microplate ReaderBio-Rad microplate reader (Model 680; Bio Rad Laboratories, Inc., Hercules, CA, USA).
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