Specific T-cell phenotype and frequencies was assessed after treatment with 10 µM SnMP in an antigen-independent setting as well as before and after expansion of WT1-specific T cells. To distinguish between naïve T cells (TN, CD62L+ CD45RA+), central memory T cells (TCM, CD62L+ CD45RA−), effector memory T cells (TEM, CD62L− CD45RA−) or terminal differentiated effector memory T cells (TEMRA, CD62L− CD45RA+), cells were stained with peridinin chlorophyll (PerCP)-conjugated anti-CD3, allophycocyanin (APC)-conjugated anti-CD8, fluorescein isothiocyanate (FITC)-conjugated anti-CD19, APC/Cyanin 7 (Cy7)-conjugated anti-CD62L and anti-CD45RA-phycoerythrin (PE)/Cy7 (all BioLegend) and analyzed by multicolor flow cytometry (FACSCanto II, FACSDiva V8.1.2 software, BD Biosciences). Gates were set on the properties regarding light scatter of lymphocytes. At least 50,000 events were acquired in the CD3+ gate.
Allophycocyanin apc conjugated anti cd8
Manufactured by BioLegend
Sourced in United States
Allophycocyanin (APC)-conjugated anti-CD8 is a fluorescent-labeled antibody that binds to the CD8 surface marker. APC is a fluorescent dye that can be detected using flow cytometry.
Automatically generated - may contain errors
Lab products found in correlation
Percp conjugated anti cd3, by BioLegend (1 mentions)
Fitc conjugated anti cd19, by BioLegend (1 mentions)
Facsdiva v8, by BD (1 mentions)
Facscanto 2, by BD (1 mentions)
Pe cy7 conjugated anti il 4, by Thermo Fisher Scientific (1 mentions)
Pe conjugated anti icos, by BioLegend (1 mentions)
Fluorescein isothiocyanate fitc conjugated anti cd4, by BioLegend (1 mentions)
Brilliant violet 421 conjugated donkey anti rabbit igg, by BioLegend (1 mentions)
Percp cy5.5 conjugated anti ifn γ, by BioLegend (1 mentions)
2 protocols using allophycocyanin apc conjugated anti cd8
1
Phenotypic Analysis of WT1-specific T Cells
2
Multiparametric Flow Cytometry Immunophenotyping
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Fluorescein isothiocyanate (FITC)-conjugated anti-CD4, allophycocyanin (APC)-conjugated anti-CD8, APC-Cy7-conjugated anti-HLA-DR, phycoerythrin (PE)-conjugated anti-ICOS, peridinin chlorophyll protein (PerCP)-conjugated anti-CD45RA, PerCP-Cy5.5-conjugated anti-IFN-γ, Brilliant Violet 421-conjugated donkey anti-rabbit IgG, and APC-Cy7/PE-Cy7-conjugated anti-CD3 were purchased from BioLegend (San Diego, CA, USA); Brilliant Violet 510-conjugated anti-IL-17 was purchased from BD Biosciences (San Diego, CA, USA); PE-Cy7-conjugated anti-IL-4 was purchased from eBioscience (San Diego, CA, USA); and rabbit anti-human LAMP-2A was purchased from Abcam (Cambridge, UK).
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