Magna pure lc 2.0 isolation station

Manufactured by Roche

The MagNA Pure LC 2.0 isolation station is a fully automated instrument designed for the purification and extraction of nucleic acids from a variety of sample types. It utilizes magnetic bead-based technology to isolate DNA, RNA, or other biomolecules with high purity and yield.

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Lab products found in correlation

Minispin, by Eppendorf (1 mentions) Magna pure lc dna isolation kit 2, by Roche (1 mentions) Magna pure lc dna isolation kit 3, by Roche (1 mentions) Magna pure lc dna isolation kit 2 tissue, by Roche (1 mentions)

Close spelling variants of this product

MagNA Pure LC (145 citations) MagNA Pure (79 citations) MagNA Pure LC 2.0 (66 citations)

2 protocols using magna pure lc 2.0 isolation station

DNA Isolation from Microbial Samples

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The control strains were suspended in sterile 0.9% saline solution until an opacity rate of 5 was achieved according to the McFarland standard. Starter cultures were dissolved in saline solution in a ratio of 1:10. Next, 1 mL of each sample suspension was centrifuged at 3286×g for 5 min in a microcentrifuge MiniSpin (Eppendorf, Hamburg, Germany), followed by the removal of 800 μL of the supernatant. The remaining 200 μL were resuspended for further DNA isolation on the MagNA Pure LC 2.0 isolation station (Roche, Mannheim, Germany) using the MagNA Pure LC DNA Isolation Kit III (bacteria, fungi) (Roche).
To isolate DNA from fermented sausages, 50 mg of samples were taken. Then, DNA isolation was performed on the MagNA Pure LC 2.0 isolation station (Roche) using the MagNA Pure LC DNA Isolation Kit II (tissue) (Roche).

Kurbakov K.A., Konorov E.A., Minaev M.Y, & Kuznetsova O.A. (2019). Multiplex Real-Time PCR with HRM for Detection of Lactobacillus sakei and Lactobacillus curvatus in Food Samples. Food Technology and Biotechnology, 57(1), 97-104.

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DNA Isolation and Real-Time PCR for Food Products

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For DNA isolation, 50 mg of food products and control samples were taken. Then lysis and purification with chloroform were carried out using the reagents of the Sorb-GMO-B Kit (Syntol, Moscow, Russia) according to the manufacturer's instructions. Further DNA isolation was performed at the MagNA Pure LC 2.0 isolation station (Roche) using the MagNa Pure LC DNA Isolation Kit II (Tissue) (Roche, Mannheim, Germany) (Kurbakov et al., 2019). Real-time PCR was performed on an ANK-32 amplifier (Syntol, Moscow, Russia). The reaction mixture (Syntol, Moscow, Russia), with a volume of 30 μL, contained primers with a concentration of 300 nM, a probe with a concentration of 150 nM, 2.5 mM MgCl2, dNTP with a concentration of 0.25 mM each, SynTaq polymerase with a concentration of 2.5 activity units, and 5 μL of isolated DNA. The PCR reaction mode was as follows: preliminary denaturation at 95 °C, 7 min, and 35 amplification cycles (60 °C for 40 s and 95 °C for 15 s).

Вострикова Н.Л., Хвостов Д.В., Zherdev A.V., Minaev M., & Zvereva E.A. (2021). Development of a two-level control system for the analysis of the composition of meat products. Potravinarstvo Slovak Journal of Food Sciences, 15, 1005-1017.

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