Iron 2 chloride hexahydrate

A Folin–Ciocalteu’s (FC) reagent, a total phenolic content (TPC) standard, iron (II) chloride hexahydrate (98%), iron (III) chloride hexahydrate (97%), iron (II) sulphate heptahydrate (99%), hydrogen chloride (HCl, 99%), sodium acetate, 2,4,6-tris-(2-pyridyl)-s-triazine (TPTZ > 98%), sinapic acid (>97%), 2,2-diphenyl-1-picrylhydrazyl (DPPH, 97%), and formic acid were all purchased from Fisher Scientific Canada Ltd. (Ottawa, ON, Canada). Aluminum chloride (AlCl₃), sodium nitrite (NaNO₂), sodium carbonate (Na₂CO₃), sodium hydroxide (NaOH), ferrozine, and disodium ethylenediaminetetraacetic acid (Na₂EDTA) were purchased from Sigma Canada Ltd. (Mississauga, ON, Canada). Quercetin hydrate (>95%) and 2-amino-ethyl-diphenyl borate (98%) were purchased from Acros (Mississauga, ON, Canada).
Extraction reagents, including methanol (optima grade) and ethanol (analytical grade), and standard compounds for high-performance liquid chromatography (HPLC) were purchased from Sigma Canada Ltd. (Mississauga, ON, Canada).

Thiol-poly (ethylene glycol)-N-Hydroxysuccinimide and thiol-poly (ethylene glycol)-nitrilotriacetic acid (MW 3400 Da) were purchased from Biochempeg Scientific, Inc. (Watertown, MA). 2-aminoethylphosphonic acid and dopamaine hydrochloride were purchased from Sigma Aldrich, Inc. (St. Louis, MO). All organic solvents [N-methylmorpholine (99%), 200-proof ethanol (99.5%, ACS), acetone (ACS), isopropanol (ACS), tetraethylammonium hydroxide (10% in water), and N,N-dimethylformamide (99.8%)], iron (III) chloride hexahydrate, iron (II) chloride hexahydrate, regenerated cellulose dialysis tubing [6,000–8,000 Da, and 3,500 Da Molecular Weight Cut-Off (MWCO)], HEPES [4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid] buffer (1 M, pH 8), and hydrochloric acid were purchased from Fisher Scientific.