Dq fitc conjugated type 1 collagen

Recombinant human ADAM9 ectodomain and the human ADAM9 ELISA kit were purchased from R&D Systems (Minneapolis, MN). Rabbit anti-ADAM9 IgG (Ab36176) and murine anti-pancytokeratin IgG and antibodies to PMN granule markers were purchased from Abcam (Cambridge, MA). Rat anti-murine Ly6G and rat anti-murine Mac-3 IgGs were purchased from BD Pharmingen (San Jose, CA). Purified human pro-matrix metalloproteinase-8 (proMMP-8) and proMMP–9 were purchased from Chemicon International, Inc. (Temecula, CA). DQ^®-FITC-conjugated gelatin, DQ^®-FITC-conjugated type IV collagen, DQ^®-FITC-conjugated type I collagen, and goat anti-rabbit F(ab’)₂ conjugated to Alexa-488 (GAR-Alexa-488) were purchased from Invitrogen (Carlsbad, CA). FITC-conjugated particulate elastin (MESH 200–400) was purchased from ICN Biomedicals Inc (Aurora, OH). L-α-phosphatidylcholine-β-y-O-alkyl platelet activating factor (PAF) was purchased from Bachem (Torrence, CA). Optimized ADAM9 and 18S Taqman® primer and probe sets were purchased from Ambion (Austin, TX). Vectashield mounting medium with DAPI was purchased from Vector Laboratories (Burlingame, CA). The murine desmosine ELISA kit was purchase from Antibodies-Online Inc. (Atlanta, GA). All other reagents were purchased from Sigma-Aldrich (St. Louis, MO).

Goat anti-rabbit F(ab)₂ conjugated to Alexa Fluor® 488, goat anti-rabbit F(ab)₂ conjugated to Alexa Fluor® 546, goat anti-mouse F(ab)₂ conjugated to Alexa Fluor® 488, and goat anti-mouse F(ab)₂ conjugated to Alexa Fluor® 546, dye quenched (DQ) FITC-conjugated gelatin, DQ-FITC conjugated type I collagen and SYTOX™ Blue Nucleic Acid Stain were obtained from Invitrogen Life Technologies (Carlsbad, CA). Purified murine Timp-1, murine Timp-2, murine Mmp-8, and murine Mmp-9 proteins were purchased from R & D Systems (Minneapolis, MN). Full length murine Mmp-8 (residues 21–465) and murine Mmp-9 (residues 20–730) proteins and mutant murine Mmp-8 (residues 21–276) and murine Mmp-9 (residues 20–519) proteins containing the catalytic domains and hinge regions but lacking the COOH-terminal hemopexin domains were obtained from Abnova (Taipei City, Taiwan). (7-Methoxycoumarin-4-yl)-Acetyl-Pro-Leu-Gly-Leu-(3-[2,4-dinitrophenyl]-L2,3-diaminopropionyl)-Ala-Arg-NH₂ (McaPLGLDpaAR) was purchased from CalBiochem Novabiochem Corp. (San Diego, CA). Murine hemopexin protein was purchased from Sino Biological (Wayne, PA).