Molecular weight standards for proteins

The RAW 264.7 cell line was purchased from American Type Culture Collection (ATCC® TIB-71™). C-PC was bought from Sigma-Aldrich (St. Louis, MO), dissolved in sterile water and stored at 4 °C. MTT assay kit, Alkaline phosphatase staining kit, and GAPDH antibody were also purchased from Sigma-Aldrich (St. Louis, MO). The following antibodies were bought from the company indicated in parenthesis: NFATc1 (SC-16657; Santa Cruz Biotechnology; Santa Cruz, CA), TRAP and CTSK (ab191406, ab19027; Abcam; Cambridge, United Kingdome), β3 Integrin, Caspase-3, caspase-9, c-Fos, and IκB-α (4702, 9662, 9504 S, 4384 S, 4814 S; Cell Signaling Technology; Danvers, MA), and HRP conjugated (mouse or rabbit) secondary antibodies (Santa Cruz Biotechnology; Santa Cruz, CA). Protein estimation reagents, molecular weight standards for proteins, and PAGE reagents were bought from Bio-Rad. Alizarin red solution was bought from Life-line Cell Technology (CM-0058; Fredrick, MD). Super Signal™ West Pico Chemiluminescent substrate was bought from Thermo Fisher Scientific (Waltham, Massachusetts). Rhodamine phalloidin and other chemicals were purchased from Sigma-Aldrich (St. Louis, MO).

Antibody to L-plastin (SC-16657; Goat) was bought from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA). Antibodies to GAPDH was purchased from R & D Systems (Minneapolis, MN) and Sigma (St. Louis, MO). Protein estimation reagent, molecular weight standards for proteins, and PAGE reagents were bought from Bio-Rad. Cy2- and Cy3-conjugated secondary antibodies were purchased from Jackson Immunoresearch (West Grove, PA). HRP-conjugated secondary antibodies for immunoblotting were obtained from GE Healthcare. Antibody to phosphoserine (p-Serine) was bought from Zymed laboratories (61–8100) or Millipore (AB1603). Alizarin red solution was bought from Life-line Cell Technology (CM-0058; Fredrick, MD) Rhodamine-phalloidin and other chemicals were purchased from Sigma (St. Louis, MO).

Methoxy terminated PLGA-PEG (10:5 kDa) was purchased from Polyscitech (West Lafayette, IN). Polyvinyl alcohol (PVA, 25 kDa) was purchased from Polysciences (Warrington, PA). Antibody to L-plastin (SC-16657) was purchased from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA). Phosphoserine (125277) antibody was bought from Abcam (Cambridge, MA). Recombinant TNF-α (216-TA) and MCSF (416-ML) were purchased from R&D Systems (Minneapolis, MN). Protein estimation reagent, molecular weight standards for proteins, and PAGE reagents were bought from Bio-Rad. HRP-conjugated secondary antibodies for immunoblotting were obtained from GE Healthcare. PLGA (7-17 kDa, 50:50), dichloromethane (DCM), anti-rabbit antibodies to GAPDH, Rhodamine-phalloidin, and other chemicals were purchased from Sigma-Aldrich (St. Louis, MO). sNT-LPL peptides (fluorescence-conjugated and unconjugated) are made from Genscript Co. (Piscataway (NJ)).