2-Acrylamido-2-methylpropanesulfonic acid (AMPS, 98%) from Tokyo Chemical Industry (Tokyo, Japan) and 4,4’-azobis(4-cyanopentanoic acid) (V-501, 98%) from Wako Pure Chemical (Osaka, Japan) were used as received without further purification, and 3-acrylamidopropyl trimethylammonium chloride (APTAC, 75 wt % in water) from Tokyo Chemical Industry was passed through an Aldrich (St Louis, MO, USA) disposable inhibitor removal column. Methanol was dried with molecular sieves 3A and purified by distillation. Phosphate buffered saline (PBS) was prepared by dissolving one PBS tablet (Aldrich) in predetermined amounts of water, while 4-cyanopentanoic acid dithiobenzoate (CPD) was synthesized according to a reported method [26 (link)]. Bovine serum albumin (BSA, pH 5.0–5.6 solution) from Wako Pure Chemical and fetal bovine serum (FBS) from GE Healthcare Life Sciences HyClone were used without further purification. Water was purified using an ion-exchange column. Other reagents were used as received.
2 acrylamido 2 methylpropanesulfonic acid amps
Manufactured by Tokyo Chemical Industry
Sourced in Japan
2-Acrylamido-2-methylpropanesulfonic acid (AMPS) is a chemical compound used as a monomer in the production of various polymers. It has a molecular formula of C₇H₁₃NO₃S and is a white crystalline solid. AMPS is primarily utilized as a building block for the synthesis of specialty polymers, copolymers, and other chemical derivatives.
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Lab products found in correlation
4 4 azobis 4 cyanopentanoic acid v 501, by Fujifilm (1 mentions)
Bovine serum albumin bsa, by Fujifilm (1 mentions)
Pyrene, by Fujifilm (1 mentions)
4 4 azobis 4 cyanovaleric acid v 501, by Fujifilm (1 mentions)
4 2 hydroxyethyl piperazine 1 ethanesulfonic acid hepes, by Merck Group (1 mentions)
2 oxoglutaric acid, by Fujifilm (1 mentions)
Hepes, by Merck Group (1 mentions)
3 protocols using 2 acrylamido 2 methylpropanesulfonic acid amps
1
Synthesis of Cationically Charged Hydrogels
2
Synthesis and Characterization of Ionic Polymers
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2-Acrylamido-2-methylpropanesulfonic acid (AMPS, 98%, Tokyo Chemical Industry, Tokyo, Japan), 4,4′-azobis(4-cyanovaleric acid) (V-501, 98%, Wako Pure Chemical, Osaka, Japan), tetrabutylphosphonium bromide (P4444Br, 95%, Wako Pure Chemical), and tributyl-n-octylphosphonium bromide (P4448Br, 98%, Tokyo Chemical Industry) were used as received. 4-Cyanopentanoic acid dithiobenzoate (CPD) was synthesized as previously reported [18 (link)]. Pyrene (97%, Wako Pure Chemical) was purified by recrystallization from ethanol. Methanol was dried with 3A molecular sieves and purified by distillation. Water was purified with an ion exchange column system. Other reagents were used as received.
3
Synthesis and Characterization of PAMPS Hydrogel
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2-acrylamido-2-methylpropanesulfonic acid (AMPS; Tokyo Chemical Industry Co., Ltd., Tokyo, Japan) and 2-oxoglutaric acid (Wako Pure Chemical Industries, Ltd, Osaka, Japan) were used as purchased. N,N'-methylenebisacrylamide (MBAA; Tokyo Chemical Industry Co., Ltd., Tokyo, Japan) was purified by recrystallization from ethanol. PAMPS gel was synthesized by radical polymerization using MBAA as a cross-linker and 2-oxoglutaric acid as an initiator. The monomer concentration was 1 mol/l for PAMPS, 4 mol% for the cross-linker, and 0.1 mol% for the initiator. Aqueous solution containing a monomer, cross-linker, and the initiator was injected into a cell consisting of a pair of glass plates separated by a silicone rubber. After gelation, gels were immersed in 4-(2-hydroxyethyl)piperazine-1-ethanesulfonic acid (HEPES; Sigma-Aldrich, St. Louis, MO, USA) buffer solution [NaHCO 3 1.55 x10 -2 M, HEPES 5 x 10 -3 M, NaCl 0.14 M, pH 7.4] and the HEPES solution was changed twice daily for 1 week to reach equilibrium. After sterilizing by autoclaving (120 ºC, 20 min), gel disks were punched out from a gel sheet with a hole punch having a diameter of 1.5 or 3.5 cm. The thickness of the gel disk was approximately 2 mm. The gel disks were then placed at the bottom of 6-well or 24-well PS plates.
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