Sensifast probes

Manufactured by Meridian Bioscience

SensiFAST™ Probes is a real-time PCR mix designed for the detection and quantification of DNA sequences using hydrolysis probe-based chemistry. It provides fast, specific, and sensitive real-time PCR results.

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Lab products found in correlation

Trizol reagent, by Thermo Fisher Scientific (4 mentions) Protoscript 2, by New England Biolabs (2 mentions) Rneasy mini column, by Qiagen (2 mentions) Dnase 1, by New England Biolabs (1 mentions) Realplex 4 thermocycler, by Eppendorf (1 mentions) Sensifast sybr kit, by Meridian Bioscience (1 mentions)

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2 protocols using sensifast probes

RNA Extraction and Real-Time PCR Protocol

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Trizol reagent (Thermo Fisher Scientific) was used to extract total
RNA from cells and liver tissue. RNA was digested with DNase I (NEB) and
further purified with RNeasy Mini columns (QIAGEN). 0.5 μg of total
RNA was used for the reverse transcription reaction with Protoscript II
(NEB). Quantitative PCR was performed using SensiFAST™ Probes or
SensiFAST™ SYBR kit (BIOLINE) in a realplex4 thermocycler
(Eppendorf). The primer sequences and TaqMan (Thermo Fisher Scientific)
probes used for qPCR are shown in Table S1. The
ΔΔCT method was used to calculate abundance of
transcripts.

Amano H., Chaudhury A., Rodriguez-Aguayo C., Lu L., Akhanov V., Catic A., Popov Y.V., Verdin E., Johnson H., Stossi F., Sinclair D.A., Nakamaru-Ogiso E., Lopez-Berestein G., Chang J.T., Neilson J.R., Meeker A., Finegold M., Baur J.A, & Sahin E. (2019). Telomere dysfunction induces sirtuin repression that drives telomere-dependent disease. Cell metabolism, 29(6), 1274-1290.e9.

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Quantitative Analysis of Liver Transcripts

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Total liver RNA was extracted with Trizol reagent (Thermo Fisher Scientific). RNA was then digested with DNase I (New Engeland Biolabs) and further cleaned up with RNeasy Mini columns (QIAGEN). cDNA synthesis was carried out with Protoscript II (New England Biolabs) using 0.5 μg of total RNA.
To quantify transcript levels, SensiFAST Probes or SensiFAST SYBR kit (BIOLINE) was used. The primer sequences used for qPCR are listed in Table 1. The ΔΔ CT method was used to calculate the expression levels of the transcripts.

Chronowski C., Akhanov V., Chan D., Catic A., Finegold M, & Sahin E. (2021). Fructose Causes Liver Damage, Polyploidy, and Dysplasia in the Setting of Short Telomeres and p53 Loss. Metabolites, 11(6), 394.

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