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Antibodies against vegf

Manufactured by Abcam
Sourced in United Kingdom

Antibodies against VEGF (Vascular Endothelial Growth Factor) are laboratory reagents used to detect and study the VEGF protein. VEGF is a signaling protein involved in the regulation of angiogenesis, the formation of new blood vessels. These antibodies can be used in various research applications, such as ELISA, Western blotting, and immunohistochemistry, to investigate VEGF expression and function.

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2 protocols using antibodies against vegf

1

Evaluation of Antiplatelet and Antithrombotic Agents

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Xinyue capsules (50 mg PQS/capsule) were provided by JiLin YiSheng Pharmaceutical Co. Ltd. (Jilin, China; SFDA approval certificate number: Z20030073). Enteric-coated aspirin tablets (100 mg) were obtained from Bayer Co. (Leverkusen, Germany; SFDA approval certificate number: J20080078). Clopidogrel tablets were obtained from Sanofi (Paris, France; SFDA approval certificate number: J20080090). Penicillin sodium injection (80 million IU/bottle) was purchased from North China Pharmaceutical Co. Ltd. (Hebei, China; SFDA approval certificate number: X1105313).
Triphenyltetrazolium chloride (TTC) was obtained from Sigma-Aldrich (St. Louis, MO, USA). Adenosine diphosphate (ADP) was obtained from Chrono-Log (Havertown, PA, USA). Fluorescent antibody PE-CD62p and FITC-CD61 were purchased from Bio-Rad Laboratories (Hercules, CA, USA). Thromboxane B2 (TXB2), 6-ketone prostaglandin F1α (6-keto-PGF1α), prostaglandin E2 (PGE2), tissue plasminogen activator (t-PA), and plasminogen activator inhibitor (PAI) radioimmunoassay kits were obtained from Huaying Engineering of Medicine and Biology Co. Ltd. (Beijing, China). Antibodies against COX-1 and COX-2 were obtained from Santa Cruz Biotechnology (Dallas, TX, USA). Antibodies against VEGF were obtained from Abcam (Cambridge, UK). Pentobarbital was obtained from Sinopharm Chemical Reagents (Beijing, China).
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2

Biochemical Assays for Angiogenic Factors

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Evo (cat. no. S2382; purity 99.76%; Selleck Chemicals, Houston, Texas, USA) was dissolved in dimethyl sulfoxide and phosphate buffer saline (PBS), respectively. Antibodies against HIF-1α, VE-cadherin, and MMP2 were purchased from Cell Signaling Technology, Inc (Danvers, Massachusetts, USA). Antibodies against VEGF, MMP9, and Platelet endothelial cell adhesion molecule-1 (CD31) were purchased from Abcam (Cambridge, UK). The antibody against glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was purchased from Bioworld Technology, Inc (Bloomington, Minnesota, USA). ML228 and PX478 were purchased from MedChemExpress, Inc. Cell Counting kit-8 was purchased from Dojindo Molecular Technologies, Inc (Kumamoto, Japan). Glycogen periodic-acid Schiff (PAS) stain kit was purchased from Beijing Solarbio Science & Technology Co., Ltd (Beijing, China).
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