Raltegravir

Manufactured by MedChemExpress

Sourced in United States

Raltegravir is a strand transfer integrase inhibitor that acts as a HIV-1 integrase inhibitor. It is a laboratory compound used in drug discovery and development research.

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3 protocols using raltegravir

Synthesis and Characterization of Antiviral Compounds

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Stock solutions (100 mM) of dNTPs were obtained from GE Healthcare. [γ−³²P] ATP (10 mCi/mL; 3000 Ci/mmol) was provided by Perkin Elmer. Synthetic oligonucleotides were obtained from Sigma and Integrated DNA Technologies. Nevirapine, efavirenz, etravirine and Rilpivirine were obtained from the AIDS Research and Reference Reagent Program, Division of AIDS, NIAID, NIH. Rilpivirine and etravirine were supplied to the Program by Tibotec Pharmaceuticals, Inc. Doravirine, raltegravir (potassium salt) and dolutegravir were purchased from MedChem Express LLC (Monmouth Junction, NJ, USA).

Álvarez M., Sapena-Ventura E., Luczkowiak J., Martín-Alonso S, & Menéndez-Arias L. (2021). Analysis and Molecular Determinants of HIV RNase H Cleavage Specificity at the PPT/U3 Junction. Viruses, 13(1), 131.

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Antiviral Drug Screening Protocol

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Acetaminophen (catalog number HY-66005), arbidol hydrochloride (HY-14904A), aspirin (HY-14654), atazanavir sulfate (HY-17367A), baloxavir marboxil (HY-109025), daclatasvir (HY-10466), darunavir (HY-17040), dolutegravir (HY-13238), efavirenz (HY-10572), elbasvir (HY-15789), etravirine (HY-90005), galidesivir (BCX4430, HY-18649A), ganciclovir (HY-13637), glecaprevir (HY-17634), hydroxychloroquine (HY-B1370), indomethacin (HY-14397), ledipasvir (HY-15602), lopinavir (HY-14588), losartan (HY-17512), nafamostat mesylate (HY-B0190A), nitazoxanide (HY-B0217), ombitasvir (HY-13997), oseltamivir (HY-13318), penciclovir (HY-17424), perindopril salt (HY-B0130A), raltegravir (HY-10353), remdesivir (GS-5734, HY-104077), rilpivirine (HY-10574), ritonavir (HY-90001), simeprevir (HY-10241), sofosbuvir (HY-15005), and β-d-N4-hydroxycytidine (HY-125033) were purchased from MedChemExpress (Monmouth Junction, NJ, United States). Amodiaquin dihydrochloride dihydrate (A2799), artesunate (A3731), chloroquine phosphate (PHR1258), mefloquine hydrochloride (PHR1705), peramivir trihydrate (SML2486), ribavirin (R9644), teicoplanin (T0578), and toremifene citrate salt (T7204) were purchased from Sigma-Aldrich (St, Louis, Missouri, United States). Favipiravir (T-705) was provided by the Toyama Chemical Co., Ltd. (Japan). The drug solutions were filtered through a 0.22 μM membrane and were stored at −80°C.

Bang M.S., Kim C.M., Kim D.M, & Yun N.R. (2022). Effective Drugs Against Severe Fever With Thrombocytopenia Syndrome Virus in an in vitro Model. Frontiers in Medicine, 9, 839215.

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Quantification of HIV-1 Integration in TZM-bl Cells

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TZM-bl cells were transfected with siRNAs as described above. Total DNA from infected TZM-bl cells was extracted using a DNeasy Blood and Tissue Kit (Qiagen) according to the manufacturer's instructions. Two hundred nanograms of DNA was subjected to the first round of PCR with primers Alu-forward 5′-GCCTCCCAAAGTGCTGGGATTACAG-3′ and HIV-1 Gag-reverse 5′-GTTCCTGCTATGTCACTTCC-3′. After 15 cycles of amplification, DNA products were quantified by real-time quantitative PCR (Lee et al., 2018 ) using primers targeting HIV-1 Gag (forward, 5′-TGCATGGGTAAAAGTAGTAGAAGAGA-3′; reverse, 5′-TGATAATGCTGAAA ACATGGGTA-3′). HIV-1 integrase inhibitor raltegravir (HY-10353, MedChemExpress, New Jersey, USA) was used as a positive control.

Shen W., Liu C., Hu Y., Ding Q., Feng J., Liu Z, & Kong X. (2023). Spastin is required for human immunodeficiency virus-1 efficient replication through cooperation with the endosomal sorting complex required for transport (ESCRT) protein. Virologica Sinica, 38(3), 448-458.

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