Percp cy5.5 anti mouse ly 6g

Manufactured by Thermo Fisher Scientific

PerCP-Cy5.5 anti-mouse Ly-6G is a fluorescent-conjugated antibody used for the detection and analysis of mouse Ly-6G, a cell surface antigen expressed on neutrophils. It can be used in flow cytometry applications.

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Lab products found in correlation

Apc cy7 anti mouse cd45, by Thermo Fisher Scientific (2 mentions) Facscanto 2, by BD (2 mentions) Pe anti mouse cd115, by Thermo Fisher Scientific (1 mentions) Anti mouse f4 80 apc, by Thermo Fisher Scientific (1 mentions) Pe anti mouse cd41, by BD (1 mentions)

2 protocols using percp cy5.5 anti mouse ly 6g

Neutrophil Identification and Discrimination

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Cell pellets were labeled with PerCP-Cy5.5 anti-mouse Ly-6G, PE anti-mouse CD115, APC-Cy7 anti-mouse CD45 and APC anti-Mouse F4/80 (all eBioscience). Neutrophils were identified by their typical appearance in the forward scatter-side scatter and as CD45⁺, CD115^– and PerCP- Gr1⁺ cells (S1 Fig). Within the lung, FITC-Gr1 antibody was used to distinguish between interstitial neutrophils (CD45⁺, CD115^–, PerCP- Gr1⁺, FITC-Gr1^-) and intravascular neutrophils (CD45⁺, CD115^–, PerCP- Gr1⁺, FITC-Gr1⁺). All flow cytometry studies were performed using a BD FACSCanto II (Becton Dickinson, San Jose, CA) and data were analyzed using FlowJo software (Tree Star, Ashland, OR).

Tilgner J., von Trotha K.T., Gombert A., Jacobs M.J., Drechsler M., Döring Y., Soehnlein O, & Grommes J. (2016). Aspirin, but Not Tirofiban Displays Protective Effects in Endotoxin Induced Lung Injury. PLoS ONE, 11(9), e0161218.

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Quantification of Lung Neutrophils

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Resuspended cells of digested lungs and BAL fluid were labeled with PerCP-Cy5.5 anti-mouse Ly6G (eBioscience, Cat No. 127615), APC-Cy7 anti-mouse CD45 (eBioscience, Cat No. 103115), and PE anti-Mouse CD41 (BD, Cat No. 561850). Neutrophils were identified as CD45 and Ly6G positive cells. All studies were carried out on a FACS Canto II (BD Biosciences) and data were analyzed using FlowJo software.

Pircher J., Czermak T., Ehrlich A., Eberle C., Gaitzsch E., Margraf A., Grommes J., Saha P., Titova A., Ishikawa-Ankerhold H., Stark K., Petzold T., Stocker T., Weckbach L.T., Novotny J., Sperandio M., Nieswandt B., Smith A., Mannell H., Walzog B., Horst D., Soehnlein O., Massberg S, & Schulz C. (2018). Cathelicidins prime platelets to mediate arterial thrombosis and tissue inflammation. Nature Communications, 9, 1523.

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