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Mouse monoclonal anti gephyrin 3b11

Manufactured by Synaptic Systems

Mouse monoclonal anti-gephyrin 3B11 is a laboratory product used for the detection and analysis of the gephyrin protein. Gephyrin is a key component of the inhibitory postsynaptic machinery and is involved in the clustering of glycine and GABA(A) receptors. This product can be used in various research applications, such as immunohistochemistry, immunocytochemistry, and Western blotting, to study the localization and expression of gephyrin in biological samples.

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2 protocols using mouse monoclonal anti gephyrin 3b11

1

Antibody Validation for Immunohistochemistry

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The following antibodies were used in immunohistochemistry and immunocitochemistry: anti-gephyrin Mab7a (Synaptic System Cat. No 147021), anti-VGAT rabbit or guinea pig (1:1000, Synaptic System Cat. No 131004), anti-NL2 rabbit affinity purified (1:500, Synaptic system Cat, No 129203), guinea pig anti-GABAA γ2 subunit (1:200044 (link)), biotinylated anti-guinea pig (1:200, Vector Laboratories, Cat No BA-7000). The following primary antibodies were used in immunoprecipitation and western blot analysis: mouse monoclonal anti-FLAG M2 (Sigma Cat No F1804), mouse monoclonal anti-gephyrin 3B11 (Synaptic System Cat No 147111) and rabbit polyclonal anti-NL2 (Synaptic Systems Cat. No 129202), pS/pT-P (MPM-2, Upstate Biotechnology Cat No 05-368), high affinity rat monoclonal anti-HA 3F10 (Roche), anti-GFP rabbit monoclonal (Life Technology, Cat No G10362). Validation of antibodies used in these assays can be found on the respective manufacturers’ websites.
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2

Characterization of Synaptic Proteins

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The following antibodies were used in immunohistochemistry and immunocitochemistry: anti-gephyrin Mab7a (Synaptic System Cat. No 147021), anti-VGAT rabbit or guinea pig (1:1,000, Synaptic System Cat. No 131004), anti-NL2 rabbit affinity purified (1:500, Synaptic system Cat, No 129203), guinea pig anti-GABAA γ2 subunit (1:2,000 (ref. 43 (link))), biotinylated anti-guinea pig (1:200, Vector Laboratories, Cat No BA-7000). The following primary antibodies were used in immunoprecipitation and western blot analysis: mouse monoclonal anti-FLAG M2 (Sigma Cat No F1804), mouse monoclonal anti-gephyrin 3B11 (Synaptic System Cat No 147111) and rabbit polyclonal anti-NL2 (Synaptic Systems Cat. No 129202), pS/pT-P (MPM2, Upstate Biotechnology Cat No 05-368), high affinity rat monoclonal anti-HA 3F10 (Roche), anti-GFP rabbit monoclonal (Life Technology, Cat No G10362). Validation of antibodies used in these assays can be found on the respective manufacturers' websites.
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