Rneasy kit mini kit

Manufactured by Qiagen

Sourced in Germany

The RNeasy Mini Kit is a laboratory equipment product used for the purification of total RNA from a variety of sample types. It utilizes a silica-membrane-based technology to efficiently capture and purify RNA molecules.

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Lab products found in correlation

Steponeplus real time pcr system, by Thermo Fisher Scientific (2 mentions) Transstart sybr green qpcr supermix, by Transgene (1 mentions) Random primers, by Vazyme (1 mentions) Iscript cdna synthesis kit, by Bio-Rad (1 mentions)

Close spelling variants of this product

RNeasy Mini KitTM RNeasy Plant Mini Kits (50) RNeasy and miRNAeasy Mini kits QIA-Shredder and RNeasy mini-kits RNeasy Mini Total RNA Purification kits RNeasy Mini Kit RNeasy® Mini Kit with RNase-free DNase treatment DNase I and RNeasy Mini kit RNeasy Mini Kit 50 RNeasy Mini Kit for mRNA

2 protocols using rneasy kit mini kit

Gene Expression Analysis by RT-qPCR

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Following the manufacturer’s instructions, total RNA was extracted by the RNeasy kit Mini Kit (Qiagen, Dusseldorf, Germany). Briefly, 2μg RNA was transformed into cDNA by reverse transcription reaction with random primers (Vazyme, Nanjing, China). The qPCR was performed on cDNA using TransStart^® SYBR Green qPCR SuperMix (TransGen, Beijing, China) on a StepOnePlus™ Real-Time PCR System (Thermo Fisher Scientific). The primers were listed as follows: BASP1 forward: 5’-GCCCAGGAGACCAAAAGTGA-3’, BASP1 reverse: 5’-CCTTGGGTGTGGAACTAGGC-3’; GAPDH forward: 5’-CTCCTCCTGTTCGACAGTCAGC-3’, GAPDH reverse: 5’-CCCAATACGACCAAATCCGTT-3’. GAPDH was used as the endogenous control. The ^ΔΔCt was used to calculate the relative mRNA expression.

Pan X., Xu X., Wang L., Zhang S., Chen Y., Yang R., Chen X., Cheng B., Xia J, & Ren X. (2023). BASP1 is a prognostic biomarker associated with immunotherapeutic response in head and neck squamous cell carcinoma. Frontiers in Oncology, 13, 1021262.

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RNA Extraction and qPCR Analysis

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RNA was extracted from exponentially growing cells using the RNeasy kit Mini Kit from Qiagen according to manufacturer’s instructions. 2 μg of total RNA was put into reverse transcription reactions using random primers for amplification (iScript™ cDNA Synthesis Kit-Bio-Rad). qPCR reactions were performed on the 7900HT Fast Real-Time PCR System using gene specific TaqMan probes or specific primers in a SYBR GREEN reaction to detect expression levels. Hypoxanthine-guanine phosphoribosyltransferase (HPRT) expression was used for normalization. The following TaqMan probes were used: Acsl3: Mm01255804_m1; Lpcat3: Mm00520147_m1; Pparγ: Mm00440940_m1; Hprt: Mm00446968_m1. The following primers were used for Acsl5 (Forward: 5′-CTGATCTGCCTCCTGACGTTTGGAA-3′; Reverse: 5′-ACAACGTCTTGGCGTCTGAGAAGT-3′) and Hprt (Forward: 5′-AGCTACTGTAATGATCAGTCAACG-3′; Reverse: 5′-AGAGGTCCTTTTCACCAGCA-3′).

Doll S., Proneth B., Tyurina Y.Y., Panzilius E., Kobayashi S., Ingold I., Irmler M., Beckers J., Aichler M., Walch A., Prokisch H., Trümbach D., Mao G., Qu F., Bayir H., Füllekrug J., Scheel C., Wurst W., Schick J.A., Kagan V.E., Angeli J.P, & Conrad M. (2016). Acsl4 Dictates Ferroptosis Sensitivity by Shaping Cellular Lipid Composition. Nature chemical biology, 13(1), 91-98.

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