Cells were cultured in 10 cm plates in a humidified atmosphere (5% CO2) at 37 °C. At 70–90% confluence, trypsin (0.25%)/EDTA solution (Corning) was used to detach the cells from the culture plate for passaging and used for further experiments until passage 20. OV81.2 and OV81.2-CP10 primary cell line models were generated as previously described [10 (link)]. A2780 and CP70 cells were obtained from Dr. Paul Modrich (Duke University). OVCAR3 cells were purchased from ATCC. OV81.2, OV81.2-CP10, A2780, CP70, ALDHhigh CP70, ALDHlow CP70, OVCAR8 and 293 T cells were cultured in DMEM medium supplemented with 10% FBS (Gibco) and 1% penicillin-streptomycin (PS) (Gibco). OVCAR3 and OVCAR3-CP38 cells were cultured in RPMI medium (Gibco) with 10% FBS and 1%PS. Phosphate-buffered saline (PBS) was purchased from Corning. Amiodarone, dronedarone, ibutilide and sotalol were purchased from SelleckChem. Dimethyl sulfoxide (DMSO) was purchased from Fisher Chemical.
Sotalol
Manufactured by Selleck Chemicals
Sotalol is a lab equipment product used for the analysis and measurement of chemical compounds. It serves as a specialized tool for researchers and scientists working in various fields of chemistry and biochemistry.
Automatically generated - may contain errors
Lab products found in correlation
Dronedarone, by Selleck Chemicals (1 mentions)
Dimethyl sulfoxide (dmso), by Thermo Fisher Scientific (1 mentions)
Amiodarone, by Selleck Chemicals (1 mentions)
Rpmi medium, by Thermo Fisher Scientific (1 mentions)
Ovcar 3 cells, by American Type Culture Collection (1 mentions)
Penicillin streptomycin p s, by Thermo Fisher Scientific (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Phosphate buffered saline (pbs), by Corning (1 mentions)
Nifedipine, by Merck Group (1 mentions)
Cilostazol, by Selleck Chemicals (1 mentions)
Quinidine, by Merck Group (1 mentions)
Bepridil, by Bio-Techne (1 mentions)
4 aminopyridine 4 ap, by Merck Group (1 mentions)
Verapamil, by Merck Group (1 mentions)
2 protocols using sotalol
1
Ovarian Cancer Cell Culture Protocol
2
Electrophysiological Experiments: Reagent Preparation
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All the chemicals used in the electrophysiological experiments were purchased from Sigma–Aldrich. Nifedipine was purchased from Sigma–Aldrich, and stock solutions were prepared in 1 mM in DMSO. 4-aminopyridine (4-AP) was purchased from Sigma–Aldrich, and stock solutions were prepared in 1 M in distilled water. Tetrodotoxin (TTX) was purchased from Absin, and stock solutions were prepared in 10 mM in distilled water. The solutions of caffeine (Sigma–Aldrich, C0750) were freshly prepared when using. Quinidine and verapamil were purchased from Sigma–Aldrich, and stock solutions were both prepared in 10 mM in DMSO. Sotalol and cilostazol were purchased from Selleck, and stock solutions were both prepared in 10 mM in DMSO. Bepridil was purchased from Tocris, and stock solutions were prepared in 1 mM in DMSO.
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