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Firstchoice rlm 5 race kit

Manufactured by Thermo Fisher Scientific
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The FirstChoice RLM-5'-RACE kit is a molecular biology tool designed to facilitate the rapid amplification of 5' cDNA ends. It provides a method for obtaining the complete 5' end sequence of mRNA transcripts.

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Lab products found in correlation

Ta vector, by Thermo Fisher Scientific (2 mentions) Pentr, by Thermo Fisher Scientific (1 mentions) T vector, by Thermo Fisher Scientific (1 mentions)

4 protocols using firstchoice rlm 5 race kit

1

5'-RACE Identification of Pst avrRpt2 Target

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5′-RACE69 (link) was performed using FirstChoice RLM-5′-RACE kit (Ambion) following the manufacturer's instructions. Briefly, total RNA was extracted from Pst (avrRpt2)-infected tissue 10 h.p.i. and directly ligated to the RNA Oligo adaptor without further modification. Oligo (dT) primer was used to prime cDNA synthesis with reverse transcriptase. Gene-specific 5′-RACE reactions were done with the 5′ Nested Primer and gene-specific primers ARLPK1-R 5′-RACE and ARLPK2-R 5′-RACE as listed in Supplementary Table 1. The 5′-RACE products were gel purified, cloned into pENTR (Invitrogen) vector and sequenced.
Niu D., Lii Y.E., Chellappan P., Lei L., Peralta K., Jiang C., Guo J., Coaker G, & Jin H. (2016). miRNA863-3p sequentially targets negative immune regulator ARLPKs and positive regulator SERRATE upon bacterial infection. Nature Communications, 7, 11324.
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2

Determination of HBL1 Transcript Sequence

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To obtain the full-length sequence of HBL1 transcript, 5 prime and 3 prime Rapid Amplification of cDNA Ends (RACE) methods (FirstChoice RLM 5′RACE kit and 3′RACE kit, Ambion) were used. Briefly, total RNA was extracted from undifferentiated human iPSCs, converted into first strand cDNA using reverse transcriptase. cDNAs were amplified, added adapters and cloned into T-vector (invitrogen). The 5′ and 3′ sequences of HBL1 were verified by Sanger sequencing.
Liu J., Li Y., Lin B., Sheng Y, & Yang L. (2017). HBL1 Is a Human Long Noncoding RNA that Modulates Cardiomyocyte Development from Pluripotent Stem Cells by Counteracting miR-1. Developmental cell, 42(4), 333-348.e5.
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3

Rapid Amplification of 5' cDNA Ends

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5’ RACE was performed using FirstChoice 5’ RLM-RACE kit
(Life Technologies). Briefly, in vitro reaction products from linearized
pLac-Tet plasmid with or without extracts were treated with Calf Intestinal
Phosphatase (CIP) and Tobacco Acid Pyrophosphatase (TAP), followed by ligation
with an adaptor, following the manufacturer’s protocol. The products were
reverse transcribed, PCR-amplified using primers (Supplementary Table 1)
matching the sequences outside the Lac and Tet repeats, cloned into TA vector
(Invitrogen) and sequenced by Sanger method.
Michelini F., Pitchiaya S., Vitelli V., Sharma S., Gioia U., Pessina F., Cabrini M., Wang Y., Capozzo I., Iannelli F., Matti V., Francia S., Shivashankar G.V., Walter N.G, & d’Adda di Fagagna F. (2017). Damage-induced lncRNAs control the DNA damage response through interaction with DDRNAs at individual double-strand breaks. Nature cell biology, 19(12), 1400-1411.
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4

Rapid Amplification of 5' cDNA Ends

Check if the same lab product or an alternative is used in the 5 most similar protocols
5’ RACE was performed using FirstChoice 5’ RLM-RACE kit
(Life Technologies). Briefly, in vitro reaction products from linearized
pLac-Tet plasmid with or without extracts were treated with Calf Intestinal
Phosphatase (CIP) and Tobacco Acid Pyrophosphatase (TAP), followed by ligation
with an adaptor, following the manufacturer’s protocol. The products were
reverse transcribed, PCR-amplified using primers (Supplementary Table 1)
matching the sequences outside the Lac and Tet repeats, cloned into TA vector
(Invitrogen) and sequenced by Sanger method.
Michelini F., Pitchiaya S., Vitelli V., Sharma S., Gioia U., Pessina F., Cabrini M., Wang Y., Capozzo I., Iannelli F., Matti V., Francia S., Shivashankar G.V., Walter N.G, & d’Adda di Fagagna F. (2017). Damage-induced lncRNAs control the DNA damage response through interaction with DDRNAs at individual double-strand breaks. Nature cell biology, 19(12), 1400-1411.
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